Western Blot (WB)
(Western blot of ERR-a antibody. Nuclear extract of MDA-MB-231 cells (20 ug per lane) probed with ERR alpha antibody at a dilution of 1:500.)
Rabbit anti-Human ERR-a Polyclonal Antibody | anti-ERR-a antibody
ERR-a antibody (pAb)
WB: 1:500-1:1,000 dilution
Western Blot (WB)
(Western blot of ERR-a antibody. Nuclear extract of MDA-MB-231 cells (20 ug per lane) probed with ERR alpha antibody at a dilution of 1:500.)
Western Blot (WB)
(Western blot of ERR-a antibody. Nuclear extract of MDA-MB-231 cells (20 ug per lane) probed with ERR alpha antibody at a dilution of 1:500.)
Background: ERR-a (Estrogen-related receptor alpha, NR3B1) is an orphan nuclear receptor similar to the Estrogen Receptor. It is a sequence-specific DNA binding transcriptional activator that is constitutively active in the absence of ligand binding. No true physiological ligands have been found, but estrogen-like compounds from plants have been shown to activate ERR-a. ERR-a and ER-a have been found to regulate an overlapping set of genes, and ERR-a appears to modulate the activity of ER-a in some tissue types. ERR-a regulates genes involved in mitochondrial biogenesis, gluconeogenesis, oxidative phosphorylation, and fatty acid metabolism. ERR-a knockout mice display impaired fat metabolism and absorption.
NCBI and Uniprot Product Information
Uniprot Description
ERR1: Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Belongs to the nuclear hormone receptor family. NR3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: microtubule cytoskeleton; nucleoplasm; intercellular bridge; nucleolus; nucleus
Molecular Function: protein domain specific binding; ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; steroid binding
Biological Process: transcription initiation from RNA polymerase II promoter; mitochondrion organization and biogenesis; intracellular receptor-mediated signaling pathway; regulation of osteoblast differentiation; organelle organization and biogenesis; negative regulation of transcription from RNA polymerase II promoter; regulation of cell proliferation; regulation of osteoclast differentiation; regulation of transcription, DNA-dependent; cartilage development; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter
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Product Notes
The ERR-an esrra (Catalog #AAA388734) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERR-an antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERR-a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 dilution. Researchers should empirically determine the suitability of the ERR-an esrra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERR-a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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