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Western Blot (WB) ((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat ERP29 Polyclonal Antibody | anti-ERP29 antibody

Goat anti-ERP29 Antibody

Gene Names
ERP29; ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
Reactivity
Tested: Human; Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ERP29; Polyclonal Antibody; Goat anti-ERP29 Antibody; endoplasmic reticulum protein 29; C12orf8; ERp28; ERp31; PDI-DB; endoplasmic reticulum lumenal protein ERp28; ERP29 antibody; endoplasmic reticulum protein 29 antibody; C12orf8 antibody; ERp28 antibody; ERp31 antibody; PDI-DB antibody; endoplasmic reticulum lumenal protein ERp28 antibody; anti-ERP29 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EKNKMSDGKKEELQ
Sequence Length
261
Applicable Applications for anti-ERP29 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx.28kDa band observed in Human Liver lysates (calculated MW of 29. 0kDa according to NP_006808.1). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-EKNKMSDGKKEELQ, from the C Terminus of the protein sequence according to NP_006808.1.
Epitope
C Terminus
Note
This antibody is expected to recognize isoform 1 (NP_006808.1) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
5,477 Da
NCBI Official Full Name
endoplasmic reticulum resident protein 29 isoform 1
NCBI Official Synonym Full Names
endoplasmic reticulum protein 29
NCBI Official Symbol
ERP29
NCBI Official Synonym Symbols
ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
NCBI Protein Information
endoplasmic reticulum resident protein 29
UniProt Protein Name
Endoplasmic reticulum resident protein 29
UniProt Gene Name
ERP29
UniProt Synonym Gene Names
C12orf8; ERP28; ERp29; ERp28; ERp31
UniProt Entry Name
ERP29_HUMAN

NCBI Description

This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

ERP29: Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome; membrane; smooth endoplasmic reticulum; transport vesicle

Molecular Function: chaperone binding; protein binding; protein disulfide isomerase activity; protein homodimerization activity

Biological Process: activation of MAPK activity; intracellular protein transport; negative regulation of protein secretion; positive regulation of protein amino acid phosphorylation; protein folding; protein secretion; protein unfolding

Research Articles on ERP29

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Product Notes

The ERP29 erp29 (Catalog #AAA421910) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ERP29 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ERP29 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Approx.28kDa band observed in Human Liver lysates (calculated MW of 29. 0kDa according to NP_006808.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the ERP29 erp29 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EKNKMSDGKK EELQ. It is sometimes possible for the material contained within the vial of "ERP29, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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