Rabbit anti-Human ERO1L Polyclonal Antibody | anti-ERO1L antibody

ERO1L, CT (ERO1L, ERO1-like protein alpha, Endoplasmic oxidoreductin-1-like protein, Oxidoreductin-1-L-alpha) (Biotin)

Cat. Num. AAA6296637

• Gene Names: ERO1L; ERO1A; ERO1-L; ERO1LA; ERO1-alpha; ERO1-L-alpha
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ERO1L; Polyclonal Antibody; CT (ERO1L; ERO1-like protein alpha; Endoplasmic oxidoreductin-1-like protein; Oxidoreductin-1-L-alpha) (Biotin); Oxidoreductin-1-L-alpha; anti-ERO1L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ERO1L antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ERO1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349-379 amino acids from the C-terminal region of human ERO1L.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ERO1L Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ERO1L antibody detected the ERO1L protein (arrow).)

Immunohistochemistry (IHC)

(ERO1L Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ERO1L Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ERO1L antibody

Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.

Product Categories/Family for anti-ERO1L antibody

Antibodies; Proteins

References

Inaba, K., et al. EMBO J. 29(19):3330-3343(2010). Appenzeller-Herzog, C., et al. EMBO J. 29(19):3318-3329(2010). Swiatkowska, M., et al. J. Biol. Chem. 285(39):29874-29883(2010). Chambers, J.E., et al. J. Biol. Chem. 285(38):29200-29207(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 7657069
• NCBI GeneID: 30001
• NCBI Accession #: NP_055399.1
• NCBI GenBank Nucleotide #: NM_014584.2
• UniProt Accession #: Q96HE7; Q7LD45; Q9P1Q9; Q9UKV6; A8K9X4; A8MYW1
• Molecular Weight: 54,393 Da
• NCBI Official Full Name: ERO1-like protein alpha
• NCBI Official Synonym Full Names: ERO1-like (S. cerevisiae)
• NCBI Official Symbol: ERO1L
• NCBI Official Synonym Symbols: ERO1A; ERO1-L; ERO1LA; ERO1-alpha; ERO1-L-alpha
• NCBI Protein Information: ERO1-like protein alpha; oxidoreductin-1-L-alpha; endoplasmic oxidoreductin-1-like protein
• UniProt Protein Name: ERO1-like protein alpha
• Protein Family: Ero1-like protein
• UniProt Gene Name: ERO1A
• UniProt Synonym Gene Names: ERO1-L; ERO1-L-alpha

Uniprot Description

Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1.