Rabbit ERK3 Polyclonal Antibody | anti-ERK3 antibody

ERK3 (Phospho-S189) Polyclonal Antibody

Cat. Num. AAA3007849

• Gene Names: MAPK6; ERK3; PRKM6; p97MAPK; HsT17250
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ERK3; Polyclonal Antibody; ERK3 (Phospho-S189) Polyclonal Antibody; Mitogen-activated protein kinase 6; MAP kinase 6; MAPK 6; Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK; MAPK6; PRKM6; anti-ERK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: P-ERK3(S189) polyclonal antibody detects endogenous levels of ERK3 protein only when phosphorylated at Ser189.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 721

• Applicable Applications for anti-ERK3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Synthetic phosphopeptide derived from human ERK3 around the phosphorylation site of Serine 189.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody.)

Related Product Information for anti-ERK3 antibody

Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK). Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl2 and caspase 3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK3 gene maps to chromosome 15q21 and encodes a 721 amino acid protein.

NCBI and Uniprot Product Information

• NCBI GI #: 4506091
• NCBI GeneID: 5597
• NCBI Accession #: NP_002739.1
• NCBI GenBank Nucleotide #: NP_002739.1
• UniProt Accession #: Q16659
• Molecular Weight: ~ 120kDa
• NCBI Official Full Name: mitogen-activated protein kinase 6
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 6
• NCBI Official Symbol: MAPK6
• NCBI Official Synonym Symbols: ERK3; PRKM6; p97MAPK; HsT17250
• NCBI Protein Information: mitogen-activated protein kinase 6
• UniProt Protein Name: Mitogen-activated protein kinase 6
• UniProt Gene Name: MAPK6
• UniProt Synonym Gene Names: ERK3; PRKM6; MAP kinase 6; MAPK 6; ERK-3; p97-MAPK
• UniProt Entry Name: MK06_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008]

Uniprot Description

ERK3: a protein kinase of the MAPK family. Plays a critical role in the regulation of cell growth and differentiation. Localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Protein type: Kinase, protein; Protein kinase, CMGC; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily

Chromosomal Location of Human Ortholog: 15q21

Cellular Component: cytoplasm; nucleus

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; protein heterodimerization activity; protein kinase binding; ATP binding

Biological Process: MAPKKK cascade; signal transduction; cell cycle; protein amino acid phosphorylation