Rabbit ERK 1/2 Polyclonal Antibody
Anti-ERK1/2 Polyclonal Antibody
Applications
Immunoblot, Western Blot
Synonyms
ERK 1/2; Polyclonal Antibody; Anti-ERK1/2 Polyclonal Antibody; anti-ERK 1/2 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
This antibody recognizes human, mouse, rat, bovine, chicken, sheep, Drosophila, and Xenopus ERK1/2.
Form/Format
Serum
Applicable Applications for anti-ERK 1/2 antibody
Immunoblot
Application Notes
Immunoblotting: use at 1:5,000 dilution. Bands of ~44 kDa (ERK1) and ~42 kDa (ERK2) are detected.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: HeLa cell lysate.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: HeLa cell lysate.
Antigen
A 35 amino acid synthetic peptide corresponding to ERK1 conjugated to KLH.
Preservatives
None.
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system
Appearance
Yellowish liquid
Preparation and Storage
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
Related Product Information for anti-ERK 1/2 antibody
The extracellular signal-regulated kinases 1 and 2 (ERK1/2) are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. The 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, so they are referred to collectively as ERK1/2. They are both found in the cytosol and mitochondria. ERK 1 and 2 are activated by MEK1 or MEK2 by dual phosphorylation of a threonine and tyrosine residue in the activation loop. Once activated, these kinases phosphorylate a variety of substrates including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases. ERK1/2 activation is believed to confer a survival advantage to cells but may also contribute to cell death under certain conditions.
Product Categories/Family for anti-ERK 1/2 antibody
Similar Products
Product Notes
The ERK 1/2 (Catalog #AAA194442) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ERK 1/2 can be used in a range of immunoassay formats including, but not limited to, Immunoblot. Immunoblotting: use at 1:5,000 dilution. Bands of ~44 kDa (ERK1) and ~42 kDa (ERK2) are detected. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate. Researchers should empirically determine the suitability of the ERK 1/2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK 1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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