Rabbit anti-Human ERI1 Polyclonal Antibody | anti-ERI1 antibody

ERI1, NT (ERI1, 3'EXO, THEX1, 3'-5' exoribonuclease 1, 3'-5' exonuclease ERI1, Eri-1 homolog, Histone mRNA 3'-end-specific exoribonuclease, Histone mRNA 3'-exonuclease 1, Protein 3'hExo) (AP)

Cat. Num. AAA6296514

• Gene Names: ERI1; HEXO; THEX1; 3'HEXO
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ERI1; Polyclonal Antibody; NT (ERI1; 3'EXO; THEX1; 3'-5' exoribonuclease 1; 3'-5' exonuclease ERI1; Eri-1 homolog; Histone mRNA 3'-end-specific exoribonuclease; Histone mRNA 3'-exonuclease 1; Protein 3'hExo) (AP); Protein 3'hExo; anti-ERI1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ERI1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ERI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-109 amino acids from the N-terminal region of human ERI1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ERI1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ERI1 antibody detected the ERI1 protein (arrow).)

Related Product Information for anti-ERI1 antibody

RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

Product Categories/Family for anti-ERI1 antibody

Antibodies; Histones

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Mullen, T.E., et al. Genes Dev. 22(1):50-65(2008). Kupsco, J.M., et al. RNA 12(12):2103-2117(2006). Yang, X.C., et al. J. Biol. Chem. 281(41):30447-30454(2006). Cheng, Y., et al. J. Mol. Biol. 343(2):305-312(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 31543184
• NCBI GeneID: 90459
• NCBI Accession #: NP_699163.2
• NCBI GenBank Nucleotide #: NM_153332.3
• UniProt Accession #: Q8IV48; Q9NSX3; A8K4U7
• Molecular Weight: 40,064 Da
• NCBI Official Full Name: 3'-5' exoribonuclease 1
• NCBI Official Synonym Full Names: exoribonuclease 1
• NCBI Official Symbol: ERI1
• NCBI Official Synonym Symbols: HEXO; THEX1; 3'HEXO
• NCBI Protein Information: 3'-5' exoribonuclease 1; Eri-1 homolog; protein 3'hExo; 3' exoribonuclease; 3'-5' exonuclease ERI1; histone mRNA 3'-exonuclease 1; three prime mRNA exonuclease 1; three prime histone mRNA exonuclease 1; histone mRNA 3' end-specific exonuclease; histone mR
• UniProt Protein Name: 3'-5' exoribonuclease 1
• Protein Family: ERI1 exoribonuclease
• UniProt Gene Name: ERI1
• UniProt Synonym Gene Names: 3'EXO; THEX1; HEXO
• UniProt Entry Name: ERI1_HUMAN

Uniprot Description

THEX1: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication- dependent histone pre-mRNAs. Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1. Interacts in a cooperative manner with SLBP to the mature 3'-end of histone mRNAs. Binds to 40S and 60S ribosomal subunits and to 80S assembled ribosomes. Found in a ternary complex with SLBP and the stem-loop structure of the 3'-end of histone mRNAs. Although it can bind simultaneously with SLBP to the 3'-end of histone mRNA, the presence of SLBP prevents the exonuclease activity.

Protein type: Hydrolase; EC 3.1.-.-; Nucleolus

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: rRNA binding; 3'-5'-exoribonuclease activity; metal ion binding; ribosome binding; 3'-5' exonuclease activity

Biological Process: RNA-mediated gene silencing; exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); rRNA 3'-end processing