Western Blot (WB)
(Western blot analysis of ERGIC3 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). ERGIC3 (arrow) was detected using the purified Pab.)
Rabbit anti-Human ERGIC3 Polyclonal Antibody | anti-ERGIC3 antibody
ERGIC3, NT (ERGIC3, C20orf47, ERV46, SDBCAG84, Endoplasmic reticulum-Golgi intermediate compartment protein 3, Serologically defined breast cancer antigen NY-BR-84) (APC)
Gene Names
ERGIC3; Erv46; CGI-54; PRO0989; C20orf47; NY-BR-84; SDBCAG84; dJ477O4.2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ERGIC3; Polyclonal Antibody; NT (ERGIC3; C20orf47; ERV46; SDBCAG84; Endoplasmic reticulum-Golgi intermediate compartment protein 3; Serologically defined breast cancer antigen NY-BR-84) (APC); Serologically defined breast cancer antigen NY-BR-84; anti-ERGIC3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-ERGIC3 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ERGIC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human ERGIC3.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ERGIC3 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). ERGIC3 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ERGIC3 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). ERGIC3 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ERGIC3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ERGIC3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB)
(Western blot analysis of ERGIC3 (arrow) using rabbit polyclonal ERGIC3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ERGIC3 gene.)
Western Blot (WB)
(Western blot analysis of ERGIC3 (arrow) using rabbit polyclonal ERGIC3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ERGIC3 gene.)
Related Product Information for anti-ERGIC3 antibody
ERGIC3 is possible role in transport between endoplasmic reticulum and Golgi.
Product Categories/Family for anti-ERGIC3 antibody
References
Nishikawa, M., et.al., Gene 386 (1-2), 42-51 (2007). Breuza, L., et.al., J. Biol. Chem. 279 (45), 47242-47253 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,222 Da
NCBI Official Full Name
endoplasmic reticulum-Golgi intermediate compartment protein 3 isoform b
NCBI Official Synonym Full Names
ERGIC and golgi 3
NCBI Official Symbol
ERGIC3
NCBI Official Synonym Symbols
Erv46; CGI-54; PRO0989; C20orf47; NY-BR-84; SDBCAG84; dJ477O4.2
NCBI Protein Information
endoplasmic reticulum-Golgi intermediate compartment protein 3; endoplasmic reticulum-localized protein ERp43; serologically defined breast cancer antigen 84; serologically defined breast cancer antigen NY-BR-84
UniProt Protein Name
Endoplasmic reticulum-Golgi intermediate compartment protein 3
Protein Family
UniProt Gene Name
ERGIC3
UniProt Synonym Gene Names
C20orf47; ERV46; SDBCAG84
UniProt Entry Name
ERGI3_HUMAN
Uniprot Description
SDBCAG84: Possible role in transport between endoplasmic reticulum and Golgi. Belongs to the ERGIC family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; Motility/polarity/chemotaxis; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 20pter-q12
Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; membrane; integral to membrane
Biological Process: vesicle-mediated transport
Research Articles on ERGIC3
Similar Products
Product Notes
The ERGIC3 ergic3 (Catalog #AAA6296493) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERGIC3, NT (ERGIC3, C20orf47, ERV46, SDBCAG84, Endoplasmic reticulum-Golgi intermediate compartment protein 3, Serologically defined breast cancer antigen NY-BR-84) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERGIC3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ERGIC3 ergic3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERGIC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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