Rabbit anti-Human ERG25 Polyclonal Antibody | anti-ERG25 antibody

ERG25, CT (MSMO1, DESP4, ERG25, SC4MOL, Methylsterol monooxygenase 1, C-4 methylsterol oxidase) (PE)

Cat. Num. AAA6296480

• Gene Names: MSMO1; DESP4; ERG25; MCCPD; SC4MOL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ERG25; Polyclonal Antibody; CT (MSMO1; DESP4; SC4MOL; Methylsterol monooxygenase 1; C-4 methylsterol oxidase) (PE); MSMO1; C-4 methylsterol oxidase; anti-ERG25 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 293

• Applicable Applications for anti-ERG25 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ERG25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-293 amino acids from the C-terminal region of human ERG25.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ERG25 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ERG25 antibody detected the ERG25 protein (arrow).)

Immunohistochemistry (IHC)

(ERG25 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tisssue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ERG25 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ERG25 antibody

Sterol-C4-mehtyl oxidase-like protein was isolated based on its similarity to the yeast ERG25 protein. It contains a set of putative metal binding motifs with similarity to that seen in a family of membrane desaturases-hydroxylases. The protein is localized to the endoplasmic reticulum membrane and is believed to function in cholesterol biosynthesis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Product Categories/Family for anti-ERG25 antibody

Antibodies; Enzymes; Oxidase

References

Lu, Y., et al. J. Lipid Res. 49(12):2582-2589(2008). Lim, J., et al. Cell 125(4):801-814(2006). Li, L., et al. J. Biol. Chem. 271(28):16927-16933(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 5803157
• NCBI GeneID: 6307
• NCBI Accession #: NP_006736.1; NP_001017369.1
• NCBI GenBank Nucleotide #: NP_006736.1
• UniProt Accession #: Q15800
• NCBI Official Full Name: methylsterol monooxygenase 1 isoform 1
• NCBI Official Synonym Full Names: methylsterol monooxygenase 1
• NCBI Official Symbol: MSMO1
• NCBI Official Synonym Symbols: DESP4; ERG25; MCCPD; SC4MOL
• NCBI Protein Information: methylsterol monooxygenase 1
• UniProt Protein Name: Methylsterol monooxygenase 1
• Protein Family: Methylsterol monooxygenase
• UniProt Gene Name: MSMO1
• UniProt Synonym Gene Names: DESP4; ERG25; SC4MOL
• UniProt Entry Name: MSMO1_HUMAN

NCBI Description

Sterol-C4-mehtyl oxidase-like protein was isolated based on its similarity to the yeast ERG25 protein. It contains a set of putative metal binding motifs with similarity to that seen in a family of membrane desaturases-hydroxylases. The protein is localized to the endoplasmic reticulum membrane and is believed to function in cholesterol biosynthesis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]