Rabbit anti-Human, Mouse ERF3B Polyclonal Antibody | anti-ERF3B antibody

ERF3B, ID (GSPT2, ERF3B, Eukaryotic peptide chain release factor GTP-binding subunit ERF3B, G1 to S phase transition protein 2 homolog) (AP)

Cat. Num. AAA6296459

• Gene Names: GSPT2; GST2; ERF3B
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ERF3B; Polyclonal Antibody; ID (GSPT2; Eukaryotic peptide chain release factor GTP-binding subunit ERF3B; G1 to S phase transition protein 2 homolog) (AP); GSPT2; G1 to S phase transition protein 2 homolog; anti-ERF3B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ERF3B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ERF3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223~253 amino acids from the Central region of human ERF3B.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ERF3B Antibody (Center) western blot analysis in 293 cell line and mouse stomach tissue lysates (35ug/lane).This demonstrates the ERF3B antibody detected the ERF3B protein (arrow).)

Related Product Information for anti-ERF3B antibody

ERF3B encodes a GTPase that belongs to the GTP-binding elongation factor family. The encoded protein is a polypeptide release factor that complexes with eukaryotic peptide chain release factor 1 to mediate translation termination. This protein may also be involved in mRNA stability.

Product Categories/Family for anti-ERF3B antibody

Antibodies; Binding Proteins

References

Cheng, Z., et al. Genes Dev. 23(9):1106-1118(2009). Zhouravleva, G., et al. IUBMB Life 58(4):199-202(2006). Chauvin, C., et al. Mol. Cell. Biol. 25(14):5801-5811(2005). Hoshino, S., et al. J. Biol. Chem. 274(24):16677-16680(1999). Hansen, L.L., et al. Cytogenet. Cell Genet. 86 (3-4), 250-251 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 46094014
• NCBI GeneID: 23708
• NCBI Accession #: NP_060564
• NCBI GenBank Nucleotide #: NM_018094.4
• UniProt Accession #: Q8IYD1; Q9H909; Q9NVY0; Q9NY44
• Molecular Weight: 68,883 Da
• NCBI Official Full Name: eukaryotic peptide chain release factor GTP-binding subunit ERF3B
• NCBI Official Synonym Full Names: G1 to S phase transition 2
• NCBI Official Symbol: GSPT2
• NCBI Official Synonym Symbols: GST2; ERF3B
• NCBI Protein Information: eukaryotic peptide chain release factor GTP-binding subunit ERF3B
• UniProt Protein Name: Eukaryotic peptide chain release factor GTP-binding subunit ERF3B
• UniProt Gene Name: GSPT2
• UniProt Synonym Gene Names: ERF3B; Eukaryotic peptide chain release factor subunit 3b; eRF3b
• UniProt Entry Name: ERF3B_HUMAN

NCBI Description

This gene encodes a GTPase that belongs to the GTP-binding elongation factor family. The encoded protein is a polypeptide release factor that complexes with eukaryotic peptide chain release factor 1 to mediate translation termination. This protein may also be involved in mRNA stability.[provided by RefSeq, Mar 2010]

Uniprot Description

GSPT2: Involved in translation termination in response to the termination codons UAA, UAG and UGA. May play a role as a potent stimulator of the release factor activity of ETF1. Exhibits GTPase activity, which is ribosome- and ETF1-dependent. May play a role in cell cycle progression. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Belongs to the GTP-binding elongation factor family. ERF3 subfamily.

Protein type: Hydrolase; RNA-binding; Translation

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: cytosol

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: cellular protein metabolic process; translation; mRNA catabolic process, nonsense-mediated decay; gene expression; translational termination; cell cycle