Rabbit anti-Human EREG Polyclonal Antibody | anti-EREG antibody

EREG, CT (EREG, Proepiregulin, Epiregulin) (MaxLight 750)

Cat. Num. AAA6296446

• Gene Names: EREG; ER; Ep; EPR
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: EREG; Polyclonal Antibody; CT (EREG; Proepiregulin; Epiregulin) (MaxLight 750); Epiregulin; anti-EREG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EREG.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-EREG antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 137-165 in the C-terminal region of human EREG, UniProt Accession #O14944.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) usingMBS6012336. EREG (arrow) was detected usingMBS6012336.)

Testing Data

(All lanes: MBS6012336 (1:2000) Lane 1: HepG2 whole cell lysates Lane 2: A549 whole cell lysates Lane 3: NCI-H1299 whole cell lysates Lane 4: U-87 MG whole cell lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted band size: 19kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry (FFPE) staining of EREG in human brain tissue sections usingMBS6012336. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with MBS6012336 (1:25) for 1 hour at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded colon carcinoma usingMBS6012336, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6012336 for immunohistochemistry.)

Product Categories/Family for anti-EREG antibody

Antibodies; Proteins

References

1. Ben-Ami, I., et al. Hum. Reprod. 24(1):176-184(2009). 2. Cho, M.C., et al. Biochem. Biophys. Res. Commun. 377(3):832-837(2008). 3. Lasky-Su, J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4557567
• NCBI GeneID: 2069
• NCBI Accession #: NP_001423.1
• NCBI GenBank Nucleotide #: NM_001432.2
• UniProt Accession #: O14944; Q6FH69; B2RC66
• Molecular Weight: 19,044 Da
• NCBI Official Full Name: proepiregulin preproprotein
• NCBI Official Synonym Full Names: epiregulin
• NCBI Official Symbol: EREG
• NCBI Official Synonym Symbols: ER; Ep; EPR
• NCBI Protein Information: proepiregulin
• UniProt Protein Name: Proepiregulin
• Protein Family: Proepiregulin
• UniProt Gene Name: EREG
• UniProt Synonym Gene Names: EPR
• UniProt Entry Name: EREG_HUMAN

Uniprot Description

Epiregulin: Ligand of the EGF receptor/EGFR and ERBB4. May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular region; extracellular space; integral to plasma membrane

Molecular Function: epidermal growth factor receptor binding; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: anatomical structure morphogenesis; cell-cell signaling; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; female meiosis; keratinocyte differentiation; keratinocyte proliferation; luteinizing hormone signaling pathway; MAPKKK cascade; mRNA transcription; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of smooth muscle cell differentiation; negative regulation of transcription, DNA-dependent; oocyte maturation; organ morphogenesis; ovarian cumulus expansion; ovulation; phosphoinositide-mediated signaling; positive regulation of cell proliferation; positive regulation of cytokine biosynthetic process; positive regulation of cytokine production; positive regulation of DNA replication; positive regulation of epidermal growth factor receptor activity; positive regulation of fibroblast proliferation; positive regulation of innate immune response; positive regulation of interleukin-6 biosynthetic process; positive regulation of mitosis; positive regulation of phosphorylation; positive regulation of protein kinase activity; positive regulation of smooth muscle cell proliferation; primary follicle stage, oogenesis; regulation of phosphoinositide 3-kinase cascade; wound healing