Rabbit anti-Human, Mouse ErbB3 Polyclonal Antibody | anti-ErbB3 antibody

ErbB3, NT (Receptor Tyrosine-protein Kinase erbB-3, Proto-oncogene-like Protein c-ErbB-3, Tyrosine Kinase-type Cell Surface Receptor HER3, HER3) (Azide free) (HRP)

Cat. Num. AAA6474761

• Gene Names: ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: ErbB3; Polyclonal Antibody; NT (Receptor Tyrosine-protein Kinase erbB-3; Proto-oncogene-like Protein c-ErbB-3; Tyrosine Kinase-type Cell Surface Receptor HER3; HER3) (Azide free) (HRP); anti-ErbB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ErbB3. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ErbB3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1,000; WB: 1:100-1:500; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 24-55 in the N-terminal region of human ErbB3, UniProt Accession #P21860.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse brain lysate usingMBS6009408. ErbB3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma usingMBS6009408., which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue usingMBS6009408, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-ErbB3 antibody

Antibodies; Oncoproteins

References

1. Katoh, M., et al., Biochem. Biophys. Res. Commun. 192(3):1189-1197 (1993). 2. Plowman, G.D., et al., Proc. Natl. Acad. Sci. USA 87(13):4905-4909 (1990). 3. Kraus, M.H., et al., Proc. Natl. Acad. Sci. USA 86(23):9193-9197 (1989). 4. Gang Xu, et al. Circ. Res. published 30 July 2009, 10.1161.

NCBI and Uniprot Product Information

• NCBI GI #: 54792102
• NCBI GeneID: 2065
• NCBI Accession #: NP_001005915.1
• NCBI GenBank Nucleotide #: NM_001005915.1
• UniProt Accession #: P21860; Q9BUD7; A8K6L6; B4DIK7; B4DV32; E9PDT8
• Molecular Weight: 148,098 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-3 isoform s
• NCBI Official Synonym Full Names: v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
• NCBI Official Symbol: ERBB3
• NCBI Official Synonym Symbols: HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-3
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: ERBB3
• UniProt Synonym Gene Names: HER3
• UniProt Entry Name: ERBB3_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HER3: a receptor tyrosine kinase of the EGFR family. Binds and is activated by neuregulins and NTAK. Can form homodimers or ErbB-2/ErbB-3 heterodimers. Kinase domain lacks activity but heterodimerizes with other EGFRs to transduce growth signals. May be required for HER2 activity. Elevated expression in breast and other tumors is indicative of poor outcome. A secreted form is expressed in metastatic prostate cancer Two alternatively spliced isoforms have been described.

Protein type: Oncoprotein; Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; TK group; EGFR family

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular space; basolateral plasma membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; receptor complex; lateral plasma membrane

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; protein homodimerization activity; protein heterodimerization activity; protein-tyrosine kinase activity; growth factor binding; protein tyrosine kinase activator activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide 3-kinase cascade; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; wound healing; heart development; cranial nerve development; negative regulation of cell adhesion; signal transduction; protein amino acid phosphorylation; peripheral nervous system development; negative regulation of signal transduction; regulation of cell proliferation; Schwann cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; neuron apoptosis; innate immune response; negative regulation of secretion; negative regulation of neuron apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Lethal Congenital Contracture Syndrome 2