Rabbit ERBB2 Polyclonal Antibody | anti-ERBB2 antibody

ERBB2 Polyclonal Antibody

Cat. Num. AAA8527320

• Gene Names: ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: ERBB2; Polyclonal Antibody; ERBB2 Polyclonal Antibody; CD340; HER-2; HER-2/neu; HER2; NEU; NGL; TKR1; anti-ERBB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 1225

• Applicable Applications for anti-ERBB2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100
• Immunogen: A synthetic peptide of human ERBB2
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of HeLacell line, using ERBB2 antibody.)

Related Product Information for anti-ERBB2 antibody

The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1, 5).

References

1. Muthuswamy, S.K. et al. (1999) Mol Cell Biol 19, 6845-57. 2. Qian, X. et al. (1994) Proc Natl Acad Sci USA 91, 1500-4. 3. Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4. 4. Klapper, L.N. et al. (2000) Cancer Res 60, 3384-8. 5. Kwon, Y.K. et al. (1997) J Neurosci 17, 8293-9.

NCBI and Uniprot Product Information

• NCBI GI #: 54792098
• NCBI GeneID: 2064
• NCBI Accession #: NP_001005862.1
• NCBI GenBank Nucleotide #: NM_001005862.2
• UniProt Accession #: P04626; Q14256; Q6LDV1; Q9UMK4; B2RZG3; B4DHN3; X5D2V5
• Molecular Weight: 138kDa
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-2 isoform b
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 2
• NCBI Official Symbol: ERBB2
• NCBI Official Synonym Symbols: NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-2
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-2
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: ERBB2
• UniProt Synonym Gene Names: HER2; MLN19; NEU; NGL; MLN 19

NCBI Description

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.