Rabbit anti-Human ERAS Polyclonal Antibody | anti-ERAS antibody

ERAS, NT (F66) (ERAS, HRAS2, HRASP, GTPase ERas, Embryonic stem cell-expressed Ras) (FITC)

Cat. Num. AAA6296176

• Gene Names: ERAS; HRAS2; HRASP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ERAS; Polyclonal Antibody; NT (F66) (ERAS; HRAS2; HRASP; GTPase ERas; Embryonic stem cell-expressed Ras) (FITC); Embryonic stem cell-expressed Ras; anti-ERAS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ERAS antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59~88 amino acids from the N-terminal region of human ERAS.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Fluorescent confocal image of SY5Y cells stained with ERAS (N-term) (F66) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1470d ERAS (N-term) (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.)

Western Blot (WB)

(Western blot analysis of ERAS Antibody (N-term) (F66) in HL60 cell line lysates (35ug/lane). ERAS (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (F66), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-ERAS antibody

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.

Product Categories/Family for anti-ERAS antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Kameda, T., Stem Cells 23 (10), 1535-1540 (2005). Takahashi, K., Nature 423 (6939), 541-545 (2003). Miyoshi, J., Nucleic Acids Res. 12 (4), 1821-1828 (1984).

NCBI and Uniprot Product Information

• NCBI GI #: 31559833
• NCBI GeneID: 3266
• NCBI Accession #: NP_853510.1
• NCBI GenBank Nucleotide #: NM_181532.3
• UniProt Accession #: Q7Z444
• Molecular Weight: 25,287 Da
• NCBI Official Full Name: GTPase ERas
• NCBI Official Synonym Full Names: ES cell expressed Ras
• NCBI Official Symbol: ERAS
• NCBI Official Synonym Symbols: HRAS2; HRASP
• NCBI Protein Information: GTPase ERas; E-Ras; small GTPase protein E-Ras; embryonic stem cell-expressed Ras; v-Ha-ras Harvey rat sarcoma viral oncogene homolog 2; v-Ha-ras Harvey rat sarcoma viral oncogene homolog pseudogene
• UniProt Protein Name: GTPase ERas
• Protein Family: GTPase
• UniProt Gene Name: ERAS
• UniProt Synonym Gene Names: HRAS2; HRASP; E-Ras
• UniProt Entry Name: RASE_HUMAN

NCBI Description

This gene encodes a constitutively active member of the small GTPase Ras protein family. The encoded protein activates the phosphatidylinositol 3-kinase signal transduction pathway in undifferentiated stem cells, but is not expressed in differentiated cells. This gene may be involved in cancer and chemotherapy resistance. [provided by RefSeq, Dec 2012]

Uniprot Description

ERas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells. Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric; G protein, monomeric, Ras; G protein

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: plasma membrane

Molecular Function: GTP binding

Biological Process: small GTPase mediated signal transduction