Rabbit ER-alpha Polyclonal Antibody | anti-ER-alpha antibody

Anti-ER-alpha Antibody

Cat. Num. AAA847833

• Gene Names: ESR1; ER; ESR; Era; ESRA; NR3A1
• Reactivity: Bovine, Porcine, Rat, Chicken, Mouse, Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Affinity purification
• Synonyms: ER-alpha; Polyclonal Antibody; Anti-ER-alpha Antibody; ESR; NR3A1; Estrogen receptor; ER; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; anti-ER-alpha antibody

• Host: Rabbit
• Reactivity: Bovine, Porcine, Rat, Chicken, Mouse, Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Affinity purified rabbit polyclonal antibody to detect Estrogen receptor-alpha expression in human, mouse, rat, bovine, chicken and porcine samples
• Purity/Purification: Affinity purification
• Form/Format: Colorless liquid. In 0.42% Potassium phosphate; 0.87% NaCl; pH7.3; 30% glycerol; and 0.01% sodium azide

• Applicable Applications for anti-ER-alpha antibody: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: WB: 1:500-1:1000; IHC: 1:100-1:200; IF: 1:100-1:500
• Immunogen: KLH-conjugated synthetic peptide targeting a sequence within the C-term region of human ER-alpha
• Preparation and Storage: Store at -20 degree C; The antibody solution should be gently mixed before use.; Shelf life: ~12 months

Immunocytochemistry/Immunofluorescence (ICC/IF))

Immunohistochemistry (IHC)

Western Blot (WB)

Related Product Information for anti-ER-alpha antibody

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined.

Product Categories/Family for anti-ER-alpha antibody

Primary Antibodies; Signal Transduction; Signal Transduction Antibodies (A-Z); Cancer Research; Epigenetic Regulation; Epigenetic Antibodies (A-Z); Antibodies & Supporting Tools; Primary Antibodies (A-Z); Antibodies for Epigenetics Research; Epigenetic Re

NCBI and Uniprot Product Information

• NCBI GI #: 62821794
• NCBI GeneID: 2099
• NCBI Accession #: NP_000116.2
• NCBI GenBank Nucleotide #: NM_000125.3
• UniProt Accession #: P03372; Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7
• Molecular Weight: 66,216 Da
• NCBI Official Full Name: estrogen receptor
• NCBI Official Synonym Full Names: estrogen receptor 1
• NCBI Official Symbol: ESR1
• NCBI Official Synonym Symbols: ER; ESR; Era; ESRA; NR3A1
• NCBI Protein Information: estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha delta 4 +49 isoform; nuclear receptor subfamily 3 group A member 1; estrogen
• UniProt Protein Name: Estrogen receptor
• UniProt Gene Name: ESR1
• UniProt Synonym Gene Names: ESR; NR3A1; ER
• UniProt Entry Name: ESR1_HUMAN

NCBI Description

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq, Jul 2008]

Uniprot Description

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus

Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity

Biological Process: estrogen receptor signaling pathway; positive regulation of nitric oxide biosynthetic process; uterus development; signal transduction; response to estradiol stimulus; regulation of apoptosis; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; antral ovarian follicle growth; positive regulation of nitric-oxide synthase activity; negative regulation of transcription factor activity; male gonad development; androgen metabolic process; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; gene expression; positive regulation of transcription factor activity; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling

Disease: Breast Cancer; Migraine With Or Without Aura, Susceptibility To, 1; Myocardial Infarction, Susceptibility To; Estrogen Resistance