Rabbit anti-Human, Mouse Epsin2 Polyclonal Antibody | anti-EPN2 antibody

Epsin2, NT (Epsin 2, EPS-15 Interacting Protein 2, EPN2, KIAA1065) (APC)

Cat. Num. AAA6474686

• Gene Names: EPN2; EHB21
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Epsin2; Polyclonal Antibody; NT (Epsin 2; EPS-15 Interacting Protein 2; EPN2; KIAA1065) (APC); anti-EPN2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Epsin2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-EPN2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human Epsin2 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-Epsin2 Pab is used in Western blot to detect Epsin2 in mouse brain tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-EPN2 antibody

Antibodies; Proteins

References

1. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). 2. Kikuno, R., et al., DNA Res. 6(3):197-205 (1999). 3. Rosenthal, J.A., et al., J. Biol. Chem. 274(48):33959-33965 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 156671215
• NCBI GeneID: 22905
• NCBI Accession #: NP_055779.2
• NCBI GenBank Nucleotide #: NM_014964.4
• UniProt Accession #: O95208; O95207; Q9H7Z2; Q9UPT7
• Molecular Weight: 68,482 Da
• NCBI Official Full Name: epsin-2 isoform b
• NCBI Official Synonym Full Names: epsin 2
• NCBI Official Symbol: EPN2
• NCBI Official Synonym Symbols: EHB21
• NCBI Protein Information: epsin-2; Eps15 binding protein; EPS-15-interacting protein 2
• UniProt Protein Name: Epsin-2
• UniProt Gene Name: EPN2
• UniProt Synonym Gene Names: KIAA1065
• UniProt Entry Name: EPN2_HUMAN

NCBI Description

This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

epsin 2: Plays a role in the formation of clathrin-coated invaginations and endocytosis. Belongs to the epsin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle

Molecular Function: lipid binding

Biological Process: Notch signaling pathway; in utero embryonic development; embryonic organ development; endocytosis