Rabbit anti-Human Epsin 3/EPN3 Polyclonal Antibody | anti-EPN3 antibody

Anti-Epsin 3/EPN3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101349

• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Epsin 3/EPN3; Polyclonal Antibody; Anti-Epsin 3/EPN3 Antibody; Rabbit Polyclonal; EPN3 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-EPN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human EPN3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-EPN3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Human EPN3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human EPN3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-EPN3 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:68 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-EPN3 antibody

Epsin3 (EPN3), together with Epsin1 (EPN1) and Epsin2 (EPN2), belongs to the evolutionarily conserved epsin family. While EPN1 and EPN2 are ubiquitous, EPN3 is expressed at low levels in normal tissues, except for gastric parietal cells. EPN3 expression is upregulated in wounded epithelial tissues (e.g., ulcerative colitis) exhibiting altered cell-extracellular matrix interactions. Epsin 3 (EPN3) has been identified as an oncogene in estrogen receptor-positive breast cancer and non-small cell lung cancer. Mechanistically, EPN3 drives breast tumorigenesis by increasing E-cadherin endocytosis, followed by the activation of a beta-catenin/TCF4-dependent partial epithelial-to-mesenchymal transition (EMT), followed by the establishment of a TGFbeta-dependent autocrine loop that sustains EMT. EPN3-induced partial EMT is instrumental for the transition from in situ to invasive breast carcinoma. EPN3 expression is limited to gastric parietal cells and wounded or pathological tissue rather than normal brain tissue.

References

Wang Y, et al. (2018) Overexpression of epsin 3 enhances migration and invasion of glioma cells by inducing epithelial-mesenchymal transition. Oncol Rep 40 (5): 3049-3059.Schiano Lomoriello I, et al. (2020) A self-sustaining endocytic-based loop promotes breast cancer plasticity leading to aggressiveness and pro-metastatic behavior. Nat Commun 11 (1): 3020.

NCBI and Uniprot Product Information

• NCBI GI #: 157388929
• NCBI GeneID: 55040
• NCBI Accession #: NP_060427.2
• NCBI GenBank Nucleotide #: NM_017957.2
• UniProt Accession #: Q9H201; Q9BVN6; Q9NWK2; A8K6J3; A8KAB2
• Molecular Weight: 68,222 Da
• NCBI Official Full Name: epsin-3
• NCBI Official Synonym Full Names: epsin 3
• NCBI Official Symbol: EPN3
• NCBI Protein Information: epsin-3; EPS-15-interacting protein 3
• UniProt Protein Name: Epsin-3
• UniProt Gene Name: EPN3
• UniProt Entry Name: EPN3_HUMAN

Uniprot Description

epsin 3: Belongs to the epsin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: extrinsic to plasma membrane; clathrin-coated vesicle; perinuclear region of cytoplasm; coated pit; nucleus

Molecular Function: protein binding; lipid binding