Rabbit anti-Human EPOR Polyclonal Antibody | anti-EPOR antibody

EPOR, ID (EPOR, Erythropoietin receptor) (PE)

Cat. Num. AAA6296106

• Gene Names: EPOR; EPO-R
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: EPOR; Polyclonal Antibody; ID (EPOR; Erythropoietin receptor) (PE); Erythropoietin receptor; anti-EPOR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EPOR.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-EPOR antibody: Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 329-357 in the central region of human EPOR, Swiss/UniProt Accession: P19235.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6003503. This demonstrates that MBS6003503 detected the EPOR protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis with Hela cells using MBS6003503 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis with Hela cells using MBS6003503 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EPOR antibody

Antibodies; Receptors

References

1. Lim, A.C., et al. Biochemistry 49(18):3797-3804 (2010). 2. Perrotta, S., et al. PLoS ONE 5 (8), E12015 (2010). 3. Khankin, E.V., et al. PLoS ONE 5 (2), E9246 (2010). 4. Wincewicz, A., et al. Folia Histochem. Cytobiol. 47(3):425-430 (2009). 5. Ketteler, R., et al. J. Biol. Chem. 278(4):2654-2660 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4503591
• NCBI GeneID: 2057
• NCBI Accession #: NP_000112.1
• NCBI GenBank Nucleotide #: NM_000121.3
• UniProt Accession #: P19235; Q15443; Q2M205; B2RCG4
• Molecular Weight: 55,065 Da
• NCBI Official Full Name: erythropoietin receptor
• NCBI Official Synonym Full Names: erythropoietin receptor
• NCBI Official Symbol: EPOR
• NCBI Official Synonym Symbols: EPO-R
• NCBI Protein Information: erythropoietin receptor
• UniProt Protein Name: Erythropoietin receptor
• Protein Family: Erythropoietin receptor
• UniProt Gene Name: EPOR
• UniProt Synonym Gene Names: EPO-R
• UniProt Entry Name: EPOR_HUMAN

NCBI Description

This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; extracellular region

Molecular Function: identical protein binding; protein binding; erythropoietin receptor activity

Biological Process: heart development; brain development; decidualization; signal transduction

Disease: Erythrocytosis, Familial, 1