Rabbit anti-Human EPOR Polyclonal Antibody | anti-EPOR antibody

EPOR Antibody (Center)

Cat. Num. AAA9200714

• Gene Names: EPOR; EPO-R
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: EPOR; Polyclonal Antibody; EPOR Antibody (Center); Erythropoietin receptor; EPO-R; anti-EPOR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This EPOR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-357 amino acids from the Central region of human EPOR.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.3 (varies by lot)
• Sequence Positions: 329-357
• Sequence Length: 508

• Applicable Applications for anti-EPOR antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(EPOR Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the EPOR antibody detected the EPOR protein (arrow).)

Flow Cytometry (FC/FACS)

(EPOR Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of EPOR Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-EPOR antibody

This gene encodes the erythropoietin receptor which is a
member of the cytokine receptor family. Upon erythropoietin
binding, this receptor activates Jak2 tyrosine kinase which
activates different intracellular pathways including: Ras/MAP
kinase, phosphatidylinositol 3-kinase and STAT transcription
factors. The stimulated erythropoietin receptor appears to have a
role in erythroid cell survival. Defects in the erythropoietin
receptor may produce erythroleukemia and familial erythrocytosis.
Dysregulation of this gene may affect the growth of certain tumors.
Alternate splicing results in multiple transcript variants.

Product Categories/Family for anti-EPOR antibody

Cardiovascular; Immunology; Signal Transduction

References

Lim, A.C., et al. Biochemistry 49(18):3797-3804(2010)
Perrotta, S., et al. PLoS ONE 5 (8), E12015 (2010) :
Khankin, E.V., et al. PLoS ONE 5 (2), E9246 (2010) :
Wincewicz, A., et al. Folia Histochem. Cytobiol. 47(3):425-430(2009)
Ketteler, R., et al. J. Biol. Chem. 278(4):2654-2660(2003)

NCBI and Uniprot Product Information

• NCBI GI #: 4503591
• NCBI GeneID: 2057
• NCBI Accession #: NP_000112.1
• NCBI GenBank Nucleotide #: NM_000121.3
• UniProt Accession #: P19235; Q15443; Q2M205; B2RCG4
• Molecular Weight: 55065
• NCBI Official Full Name: erythropoietin receptor
• NCBI Official Synonym Full Names: erythropoietin receptor
• NCBI Official Symbol: EPOR
• NCBI Official Synonym Symbols: EPO-R
• NCBI Protein Information: erythropoietin receptor
• UniProt Protein Name: Erythropoietin receptor
• Protein Family: Erythropoietin receptor
• UniProt Gene Name: EPOR
• UniProt Synonym Gene Names: EPO-R
• UniProt Entry Name: EPOR_HUMAN

NCBI Description

This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; extracellular region

Molecular Function: identical protein binding; protein binding; erythropoietin receptor activity

Biological Process: heart development; decidualization; brain development; signal transduction

Disease: Erythrocytosis, Familial, 1