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Rabbit anti-Human, Mouse Ephrin B1 Polyclonal Antibody | anti-Ephb1 antibody

Ephrin B1 (Ephrin-B1, ELK-L, EFL-3, Cek5-L, LERK-2)

Gene Names
Ephb1; Elk; Net; Cek6; Elkh; Hek6; AW488255; 9330129L11; C130099E04Rik; ENSMUSG00000074119
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
Ephrin B1; Polyclonal Antibody; Ephrin B1 (Ephrin-B1; ELK-L; EFL-3; Cek5-L; LERK-2); Anti -Ephrin B1 (Ephrin-B1; anti-Ephb1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Speci c for the ephrin-B1 protein.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Applicable Applications for anti-Ephb1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry.
Dilution: Western Blotting: 1-5ug/mL
Immunoprecipitation: 5-10ug/ IP reaction
Immunohistochemistry (paraf n): 5-10ug/mL (pepsin digestion was required for optimal staining)
Immunogen
Synthetic peptide derived from the internal sequence of the mouse ephrin-B1 protein
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-Ephb1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
109,881 Da
NCBI Official Full Name
Eph receptor B1
NCBI Official Synonym Full Names
Eph receptor B1
NCBI Official Symbol
Ephb1
NCBI Official Synonym Symbols
Elk; Net; Cek6; Elkh; Hek6; AW488255; 9330129L11; C130099E04Rik; ENSMUSG00000074119
NCBI Protein Information
ephrin type-B receptor 1; OTTMUSP00000023194
UniProt Protein Name
Ephrin type-B receptor 1
Protein Family
UniProt Gene Name
Ephb1
UniProt Synonym Gene Names
ENSMUSG00000074119
UniProt Entry Name
EPHB1_MOUSE

Uniprot Description

EphB1: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-B family: ephrin-B1, -B2 and -B3. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB1 interacts with GRB2, GRB10 and NCK through their respective SH2 domains. Four alternatively spliced isoforms are known.

Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; Membrane protein, integral; Protein kinase, TK; TK group; Eph family

Cellular Component: filopodium tip; cell projection; membrane; axon; early endosome membrane; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; endosome; lipid raft

Molecular Function: transmembrane-ephrin receptor activity; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; protein kinase activity; transferase activity; protein binding; protein-tyrosine kinase activity; ephrin receptor activity; transferase activity, transferring phosphorus-containing groups; protein complex binding; kinase activity; axon guidance receptor activity; ATP binding

Biological Process: axon guidance; nervous system development; peptidyl-tyrosine phosphorylation; formation of immunological synapse; protein amino acid autophosphorylation; cranial nerve development; central nervous system projection neuron axonogenesis; optic nerve morphogenesis; regulation of JNK cascade; protein amino acid phosphorylation; cell-substrate adhesion; positive regulation of synaptogenesis; camera-type eye morphogenesis; neurogenesis; establishment of cell polarity; ephrin receptor signaling pathway; angiogenesis; detection of temperature stimulus involved in sensory perception of pain; cell adhesion; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; retinal ganglion cell axon guidance

Research Articles on Ephb1

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Product Notes

The Ephb1 ephb1 (Catalog #AAA615633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ephrin B1 (Ephrin-B1, ELK-L, EFL-3, Cek5-L, LERK-2) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ephrin B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry. Dilution: Western Blotting: 1-5ug/mL Immunoprecipitation: 5-10ug/ IP reaction Immunohistochemistry (paraf n): 5-10ug/mL (pepsin digestion was required for optimal staining). Researchers should empirically determine the suitability of the Ephb1 ephb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ephrin B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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