Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using EPHB4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human, Mouse EPHB4 Polyclonal Antibody | anti-EPHB4 antibody
EPHB4 Polyclonal Antibody
Gene Names
RNF112; BFP; ZNF179
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purification
Synonyms
EPHB4; Polyclonal Antibody; EPHB4 Polyclonal Antibody; HTK; MYK1; TYRO11; anti-EPHB4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
631
Applicable Applications for anti-EPHB4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
FC: 1:20 - 1:50
IHC: 1:50 - 1:200
FC: 1:20 - 1:50
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human EPHB4
Calculated Molecular Weight
108kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using EPHB4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using EPHB4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-EPHB4 antibody
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development.
Product Categories/Family for anti-EPHB4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
632
NCBI Official Full Name
RING finger protein 112
NCBI Official Synonym Full Names
ring finger protein 112
NCBI Official Symbol
RNF112
NCBI Official Synonym Symbols
BFP; ZNF179
NCBI Protein Information
RING finger protein 112; brain finger protein; zinc finger protein 179
UniProt Protein Name
RING finger protein 112
Protein Family
UniProt Gene Name
RNF112
UniProt Synonym Gene Names
BFP; ZNF179
UniProt Entry Name
RN112_HUMAN
NCBI Description
This gene encodes a member of the RING finger protein family of transcription factors. The protein is primarily expressed in brain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Uniprot Description
RNF112: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Ubiquitin conjugating system; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 17p11.2
Cellular Component: cytoplasm; integral to membrane; nucleus
Molecular Function: GTPase activity; GTP binding; zinc ion binding
Biological Process: metabolic process; positive regulation of glial cell differentiation; positive regulation of neuron differentiation
Research Articles on EPHB4
Similar Products
Product Notes
The EPHB4 rnf112 (Catalog #AAA128738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EPHB4 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EPHB4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:200 - 1:2000 IHC: 1:50 - 1:200 FC: 1:20 - 1:50. Researchers should empirically determine the suitability of the EPHB4 rnf112 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPHB4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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