Rabbit anti-Human EphA7 Polyclonal Antibody | anti-EphA7 antibody

EphA7 (Ephrin Type-A Receptor 7, EPH Homology Kinase 3, EHK-3, EPH-like Kinase 11, EK11, hEK11, EHK3, HEK11) (AP)

Cat. Num. AAA6474311

• Gene Names: EPHA7; EHK3; HEK11
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: EphA7; Polyclonal Antibody; EphA7 (Ephrin Type-A Receptor 7; EPH Homology Kinase 3; EHK-3; EPH-like Kinase 11; EK11; hEK11; EHK3; HEK11) (AP); anti-EphA7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EphA7.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-EphA7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Recombinant human EphA7 protein.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EphA7 (arrow) using rabbit polyclonal EphA7 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA7 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EphA7 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-EphA7 antibody

Antibodies; Protein Kinases

References

1. Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001). 2. Xu, Q., et al., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 355(1399):993-1002 (2000). 3. Holder, N., et al., Development 126(10):2033-2044 (1999). 4. Zhou, R., Pharmacol. Ther. 77(3):151-181 (1998). 5. Fox, G.M., et al., Oncogene 10(5):897-905 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4758282
• NCBI GeneID: 2045
• NCBI Accession #: NP_004431.1
• NCBI GenBank Nucleotide #: NM_004440.3
• UniProt Accession #: Q15375; Q59G40; Q5VTU0; Q8N368; Q9H124; A0AUX7; B2R8W1; B7ZLJ9; B7ZLK0
• Molecular Weight: 112,097 Da
• NCBI Official Full Name: ephrin type-A receptor 7
• NCBI Official Synonym Full Names: EPH receptor A7
• NCBI Official Symbol: EPHA7
• NCBI Official Synonym Symbols: EHK3; HEK11
• NCBI Protein Information: ephrin type-A receptor 7; EK11; EHK-3; EPH-like kinase 11; EPH homology kinase 3; Eph homology kinase-3; receptor protein-tyrosine kinase HEK11; tyrosine-protein kinase receptor EHK-3
• UniProt Protein Name: Ephrin type-A receptor 7
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA7
• UniProt Synonym Gene Names: EHK3; HEK11; EHK-3; EK11; hEK11
• UniProt Entry Name: EPHA7_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Uniprot Description

EphA7: a widely-expressed receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A2, -A3, -A4 and -A5. Contains 1 sterile alpha motif (SAM) and two fibronectin type III domains.

Protein type: Protein kinase, TK; Membrane protein, integral; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Eph family

Chromosomal Location of Human Ortholog: 6q16.1

Cellular Component: postsynaptic membrane; cell soma; integral to plasma membrane; dendrite; plasma membrane; neuromuscular junction

Molecular Function: protein binding; ephrin receptor binding; chemorepellent activity; protein-tyrosine kinase activity; GPI-linked ephrin receptor activity; axon guidance receptor activity; ATP binding

Biological Process: axon guidance; regulation of cell-cell adhesion; peptidyl-tyrosine phosphorylation; branching morphogenesis of a nerve; regulation of protein amino acid autophosphorylation; positive regulation of neuron apoptosis; ephrin receptor signaling pathway; negative chemotaxis; brain development; regulation of caspase activity; phosphorylation; regulation of peptidyl-tyrosine phosphorylation; retinal ganglion cell axon guidance