Rabbit anti-Human, Mouse EphA2 Receptor Polyclonal Antibody | anti-EPHA2 antibody

EphA2 Receptor, NT (Ephrin Type-A Receptor 2, Tyrosine-protein Kinase Receptor ECK, Epithelial Cell Kinase, ECK) (MaxLight 550)

Cat. Num. AAA6474219

• Gene Names: EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: EphA2 Receptor; Polyclonal Antibody; NT (Ephrin Type-A Receptor 2; Tyrosine-protein Kinase Receptor ECK; Epithelial Cell Kinase; ECK) (MaxLight 550); anti-EPHA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EphA2 Receptor. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-EPHA2 antibody: FLISA, Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: FLISA: 1:1,000; WB: 1:100-1:500; FC/FACS: 1:10-1:50; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 30-60 in the N-terminal region of human EphA2 Receptor, UniProt Accession #P29317. Species sequence homology: monkey
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MCF-7 cell line lysates (35ug/lane) usingMBS630997. EPHA2 (arrow) was detected usingMBS630997.)

Western Blot (WB)

(Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) usingMBS630997. EPHA2 (arrow) was detected usingMBS630997.)

Western Blot (WB)

(Western Blot analysis of EPHA2 (arrow) in 293 cell lysates (2ug/lane) using MBS630997 either nontransfected (Lane 1) or transiently transfected with the EPHA2 gene (Lane 2))

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted usingMBS630997, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS630997 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H292 cells using MBS630997 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EPHA2 antibody

Antibodies; Protein Kinases

References

1. Lindberg, R.A., et al., Mol. Cell. Biol. 10(12):6316-6324 (1990).

NCBI and Uniprot Product Information

• NCBI GI #: 32967311
• NCBI GeneID: 1969
• NCBI Accession #: NP_004422.2
• NCBI GenBank Nucleotide #: NM_004431.3
• UniProt Accession #: P29317; Q8N3Z2
• Molecular Weight: 108,266 Da
• NCBI Official Full Name: ephrin type-A receptor 2
• NCBI Official Synonym Full Names: EPH receptor A2
• NCBI Official Symbol: EPHA2
• NCBI Official Synonym Symbols: ECK; CTPA; ARCC2; CTPP1; CTRCT6
• NCBI Protein Information: ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase
• UniProt Protein Name: Ephrin type-A receptor 2
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA2
• UniProt Synonym Gene Names: ECK
• UniProt Entry Name: EPHA2_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]

Uniprot Description

EphA2: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice.

Protein type: Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; Eph family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: cell surface; focal adhesion; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; ephrin receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: neural tube development; axon guidance; peptidyl-tyrosine phosphorylation; viral reproduction; multicellular organismal development; notochord formation; osteoclast differentiation; bone remodeling; regulation of blood vessel endothelial cell migration; regulation of cell adhesion mediated by integrin; protein kinase B signaling cascade; ephrin receptor signaling pathway; angiogenesis; cell adhesion; vasculogenesis; skeletal development; axial mesoderm formation; cell migration; negative regulation of protein kinase B signaling cascade; mammary gland epithelial cell proliferation; regulation of angiogenesis; keratinocyte differentiation; osteoblast differentiation; DNA damage response, signal transduction resulting in induction of apoptosis; notochord cell development

Disease: Cataract 6, Multiple Types