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Western Blot (WB) (Western Blot analysis of lysates from Hela, 293T cell line (from left to right, 20ug/lane), using MBS629889 (1:1000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Rabbit anti-Human EPHA10 Polyclonal Antibody | anti-EPHA10 antibody

EPHA10, NT (Ephrin Type-A Receptor 10) (MaxLight 650)

Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
EPHA10; Polyclonal Antibody; NT (Ephrin Type-A Receptor 10) (MaxLight 650); anti-EPHA10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human EPHA10.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Sequence Length
295
Applicable Applications for anti-EPHA10 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-1:50
FLISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 149-179 in the N-terminal region of human EPHA10, UniProt Accession #Q5JZY3. Species sequence homology: chicken, mouse, rat
Conjugate
MaxLight650
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of lysates from Hela, 293T cell line (from left to right, 20ug/lane), using MBS629889 (1:1000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Western Blot (WB) (Western Blot analysis of lysates from Hela, 293T cell line (from left to right, 20ug/lane), using MBS629889 (1:1000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS629889. EPHA10 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS629889. EPHA10 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2ug/lane) usingMBS629889, either nontransfected (Lane 1) or transiently transfected with the EPHA10 gene (Lane 2) (Origene Technologies).(2ug/ml))

Western Blot (WB) (Western Blot analysis in 293 cell lysates (2ug/lane) usingMBS629889, either nontransfected (Lane 1) or transiently transfected with the EPHA10 gene (Lane 2) (Origene Technologies).(2ug/ml))

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human testis tissue reacted withMBS629889, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629889 for immunohistochemistry.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human testis tissue reacted withMBS629889, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629889 for immunohistochemistry.)
Product Categories/Family for anti-EPHA10 antibody
References
1. Aasheim, H.C., Biochim. Biophys. Acta 1723(1-3), 1-7 (2005).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
97.205
NCBI Official Full Name
ephrin type-A receptor 10 isoform 2
NCBI Official Synonym Full Names
EPH receptor A10
NCBI Official Symbol
EPHA10
NCBI Protein Information
ephrin type-A receptor 10
UniProt Protein Name
Ephrin type-A receptor 10
Protein Family
UniProt Gene Name
EPHA10
UniProt Entry Name
EPHAA_HUMAN

NCBI Description

Ephrin receptors, the largest subfamily of receptor tyrosine kinases (RTKs), and their ephrin ligands are important mediators of cell-cell communication regulating cell attachment, shape, and mobility in neuronal and epithelial cells (Aasheim et al., 2005 [PubMed 15777695]). See MIM 179610 for additional background on Eph receptors and ephrins.[supplied by OMIM, Mar 2008]

Uniprot Description

EphA10: Receptor for members of the ephrin-A family. Binds to EFNA3, EFNA4 and EFNA5. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; transmembrane-ephrin receptor activity; ephrin receptor activity; ATP binding

Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; ephrin receptor signaling pathway

Research Articles on EPHA10

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Product Notes

The EPHA10 epha10 (Catalog #AAA6474330) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EPHA10, NT (Ephrin Type-A Receptor 10) (MaxLight 650) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EPHA10 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-1:50 FLISA: 1:1,000 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the EPHA10 epha10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPHA10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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