Rabbit EPB41 Polyclonal Antibody | anti-EPB41 antibody

EPB41 (Erythrocyte Membrane Protein Band 4.1, HE, EL1, 4.1R) (HRP)

Cat. Num. AAA6415951

• Gene Names: EPB41; HE; EL1; 4.1R
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: EPB41; Polyclonal Antibody; EPB41 (Erythrocyte Membrane Protein Band 4.1; HE; EL1; 4.1R) (HRP); anti-EPB41 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes EPB41. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-EPB41 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human EPB41.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using EPB41 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using EPB41 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-EPB41 antibody

Antibodies; Membrane Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 260436831
• NCBI GeneID: 2035
• NCBI Accession #: NP_001159477.1
• NCBI GenBank Nucleotide #: NM_001166005.1
• UniProt Accession #: P11171; P11176; Q14245; Q5TB35; Q5VXN8; Q8IXV9; Q9Y578; Q9Y579; B1ALH8; B1ALH9; D3DPM9; D3DPN0
• Molecular Weight: 97,017 Da
• NCBI Official Full Name: protein 4.1 isoform 1
• NCBI Official Synonym Full Names: erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked)
• NCBI Official Symbol: EPB41
• NCBI Official Synonym Symbols: HE; EL1; 4.1R
• NCBI Protein Information: protein 4.1; P4.1; EPB4.1; band 4.1; erythrocyte surface protein band 4.1
• UniProt Protein Name: Protein 4.1
• Protein Family: Erythroid protein
• UniProt Gene Name: EPB41
• UniProt Synonym Gene Names: E41P; P4.1
• UniProt Entry Name: 41_HUMAN

NCBI Description

The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Oct 2009]

Uniprot Description

EPB41: Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Defects in EPB41 are the cause of elliptocytosis type 1 (EL1). EL1 is a Rhesus-linked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant, hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Defects in EPB41 are a cause of hereditary pyropoikilocytosis (HPP). HPP is an autosomal recessive hematologic disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1p33-p32

Cellular Component: spectrin; cortical cytoskeleton; Golgi apparatus; protein complex; extrinsic to membrane; intracellular membrane-bound organelle; plasma membrane; nucleus

Molecular Function: calmodulin binding; protein binding; spectrin binding; structural constituent of cytoskeleton; actin binding; phosphatidylinositol binding

Biological Process: positive regulation of protein binding; blood circulation; cortical actin cytoskeleton organization and biogenesis; actin cytoskeleton organization and biogenesis

Disease: Elliptocytosis 1; Pyropoikilocytosis, Hereditary