Western Blot (WB)
(EPAS1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the EPAS1 antibody detected the EPAS1 protein (arrow).)
Rabbit anti-Human EPAS1 Polyclonal Antibody | anti-EPAS1 antibody
EPAS1 Antibody (N-term)
Western Blot (WB)
(EPAS1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the EPAS1 antibody detected the EPAS1 protein (arrow).)
Western Blot (WB)
(EPAS1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the EPAS1 antibody detected the EPAS1 protein (arrow).)
Flow Cytometry (FC/FACS)
(EPAS1 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(EPAS1 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
induction of genes regulated by oxygen, which is induced as oxygen
levels fall. The encoded protein contains a basic-helix-loop-helix
domain protein dimerization domain as well as a domain found in
proteins in signal transduction pathways which respond to oxygen
levels. Mutations in this gene are associated with erythrocytosis
familial type 4.
Yi, X., et al. Science 329(5987):75-78(2010)
Bougatef, F., et al. PLoS ONE 5 (8), E12265 (2010) :
Hossein Ghaderian, S.M., et al. Pathology 42(5):446-453(2010)
Mowat, F.M., et al. PLoS ONE 5 (6), E11103 (2010) :
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
Uniprot Description
HIF2A: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP. Interacts with EGLN1. Interacts with VHL. Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 2p21-p16
Cellular Component: nucleoplasm; transcription factor complex; cytosol
Molecular Function: histone acetyltransferase binding; protein binding; signal transducer activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; myoblast cell fate commitment; mitochondrion organization and biogenesis; embryonic placenta development; somatic stem cell maintenance; regulation of heart rate; cell maturation; norepinephrine metabolic process; signal transduction; iron ion homeostasis; visual perception; response to hypoxia; erythrocyte differentiation; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; surfactant homeostasis; lung development
Disease: Erythrocytosis, Familial, 4
Research Articles on EPAS1
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Product Notes
The EPAS1 epas1 (Catalog #AAA9205250) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 124-150. The EPAS1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EPAS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the EPAS1 epas1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPAS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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