Rabbit anti-Human Eotaxin Polyclonal Antibody
Eotaxin (CCL11, C-C Motif Chemokine 11, Eosinophil Chemotactic Protein, Small-inducible Cytokine A11, SCYA11, MGC22554) (HRP)
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Neutralization Assay, Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
Eotaxin; Polyclonal Antibody; Eotaxin (CCL11; C-C Motif Chemokine 11; Eosinophil Chemotactic Protein; Small-inducible Cytokine A11; SCYA11; MGC22554) (HRP); anti-Eotaxin antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Eotaxin.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-Eotaxin antibody
Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Neutralization, Western Blot (WB)
Application Notes
IHC-P: This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 0.50-0.75ug/ml with an overnight incubation at 4 degree C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
ELISA: To detect hEotaxin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required.
Neutralization: To yield one-half maximal inhibition of the biological activity of hEotaxin (100ng/ml), a concentration of 8.0-10ug/ml of this antibody is required.
WB: To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Applications are based on unconjugated antibody.
ELISA: To detect hEotaxin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required.
Neutralization: To yield one-half maximal inhibition of the biological activity of hEotaxin (100ng/ml), a concentration of 8.0-10ug/ml of this antibody is required.
WB: To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Applications are based on unconjugated antibody.
Immunogen
Highly purified human Eotaxin.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Product Categories/Family for anti-Eotaxin antibody
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Product Notes
The Eotaxin (Catalog #AAA6403504) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Eotaxin (CCL11, C-C Motif Chemokine 11, Eosinophil Chemotactic Protein, Small-inducible Cytokine A11, SCYA11, MGC22554) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Eotaxin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Neutralization, Western Blot (WB). IHC-P: This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 0.50-0.75ug/ml with an overnight incubation at 4 degree C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. ELISA: To detect hEotaxin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required. Neutralization: To yield one-half maximal inhibition of the biological activity of hEotaxin (100ng/ml), a concentration of 8.0-10ug/ml of this antibody is required. WB: To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 1.5-3ng/lane, under either reducing or non-reducing conditions. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Eotaxin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eotaxin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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