Western Blot (WB)
(Western Blot Analysis: Representative lot data. E13-14 mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with MBS629897 (1:10,000). Proteins were visualized using a Rabbit Anti-Chicken conjugated to HRP and a chemi- luminescence detection system. Arrow indicates EOMES (~73kD).)
Chicken EOMES Polyclonal Antibody | anti-EOMES antibody
EOMES, NT (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)
Purified by affinity chromatography.
Purified by affinity chromatography.
Dilution: Western Blot: 0.5ug/ml detected Tbr2 in 10ug of E13-14 mouse brain lysates.
Immunohistochemistry (Paraffin): 1:100 dtected Tbr2 in mouse and human brain. Requires HIER with citrate buffer pH6.0
Western Blot (WB)
(Western Blot Analysis: Representative lot data. E13-14 mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with MBS629897 (1:10,000). Proteins were visualized using a Rabbit Anti-Chicken conjugated to HRP and a chemi- luminescence detection system. Arrow indicates EOMES (~73kD).)
Western Blot (WB)
(Western Blot Analysis: Representative lot data. E13-14 mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with MBS629897 (1:10,000). Proteins were visualized using a Rabbit Anti-Chicken conjugated to HRP and a chemi- luminescence detection system. Arrow indicates EOMES (~73kD).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in paraffin-embedded mouse brain tissue was prepared using heat- induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution of MBS629897. Staining pattern appears as cytoplasmic.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in paraffin-embedded mouse brain tissue was prepared using heat- induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution of MBS629897. Staining pattern appears as cytoplasmic.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in paraffin-embedded human brain tissue was prepared using heat- induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution of MBS629897. Staining pattern appears as cytoplasmic.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in paraffin-embedded human brain tissue was prepared using heat- induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution of MBS629897. Staining pattern appears as cytoplasmic.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in paraffin-embedded mouse olfactory lobe was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using a 1:100 dilution of MBS629897. Reactivity is mostly nuclear.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in paraffin-embedded mouse olfactory lobe was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using a 1:100 dilution of MBS629897. Reactivity is mostly nuclear.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in paraffin-embedded mouse cerebellum was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using 1:100 dilution of MBS629897. Immuno- reactivity is mostly nuclear.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in paraffin-embedded mouse cerebellum was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using 1:100 dilution of MBS629897. Immuno- reactivity is mostly nuclear.)
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution of MBS629897. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit . Immunoreactivity seen here is mostly nuclear.)
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution of MBS629897. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit . Immunoreactivity seen here is mostly nuclear.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the TBR1 (T-box brain protein 1) sub-family of T-box genes that share the common DNA-binding T-box domain. The encoded protein is a transcription factor which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. The protein may also be necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. A similar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Uniprot Description
EOMES: Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Up-regulated in CD8+ T-cells simultaneously stimulated with TGFB1 and IL4/interleukin-4. Expressed in CD8+ T-cells. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 3p24.1
Cellular Component: nucleus
Molecular Function: DNA binding; sequence-specific DNA binding; chromatin binding; transcription factor activity
Biological Process: CD8-positive, alpha-beta T cell differentiation during immune response; transcription, DNA-dependent; regulation of neuron differentiation; olfactory bulb development; endodermal cell fate specification; positive regulation of transcription, DNA-dependent; stem cell maintenance; cerebral cortex regionalization; negative regulation of transcription from RNA polymerase II promoter; trophectodermal cell differentiation; cerebral cortex neuron differentiation; cardioblast differentiation; mesoderm formation; endoderm formation; interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; brain development; positive regulation of cell differentiation
Research Articles on EOMES
Similar Products
Product Notes
The EOMES eomes (Catalog #AAA629897) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The EOMES, NT (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EOMES can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry., Dilution: Western Blot: 0.5ug/ml detected Tbr2 in 10ug of E13-14 mouse brain lysates. Immunohistochemistry (Paraffin): 1:100 dtected Tbr2 in mouse and human brain. Requires HIER with citrate buffer pH6.0. Researchers should empirically determine the suitability of the EOMES eomes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EOMES, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
