Rabbit anti-Human ENPP2 Polyclonal Antibody | anti-ENPP2 antibody

ENPP2, ID (Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2, E-NPP 2, Extracellular Lysophospholipase D, LysoPLD, Autotaxin, ATX, PDNP2) (Biotin)

Cat. Num. AAA6474049

• Gene Names: ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ENPP2; Polyclonal Antibody; ID (Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2; E-NPP 2; Extracellular Lysophospholipase D; LysoPLD; Autotaxin; ATX; PDNP2) (Biotin); anti-ENPP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ENPP2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ENPP2 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 377-406 in the central region of human ENPP2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2ug/lane) using E3224-02A: Lane 1: nontransfected Lane 2: transiently transfected with the ENPP2 gene.)

Western Blot (WB)

(Western Blot analysis of lysates from human placenta and plasma tissue lysate (from left to right, 35ug/lane), using MBS629986 (1:1000/lane). An IgG (HRP) goat anti-rabbit was used as the secondary antibody.)

Western Blot (WB)

(Western Blot analysis in 293T cell lysates (2ug/lane) usingMBS629986. Lane 1: nontransfected Lane 2: transiently transfected with the gene.)

Western Blot (WB)

(Western Blot analysis in human brain lysate (20ug) using MBS629986 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 99kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of MDA-MB435 cells using E3324-02A (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ENPP2 antibody

ENPP2 functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This protein stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The protein is secreted and further processed to make the biologically active form.

Product Categories/Family for anti-ENPP2 antibody

Antibodies; Enzymes; Phosphatase

References

1. Kawagoe H., Soma O., Goji J., Nishimura N., Narita M., Genomics 30:380-384(1995) 2. Nam S.W., Clair T., Kim Y.S., McMarlin A., Cancer Res. 61:6938-6944(2001). 3. The MGC Project Team Genome Res. 14:2121-2127(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 290457674
• NCBI GeneID: 5168
• UniProt Accession #: Q13822; Q13827; Q14555; Q15117; Q9UCQ8; Q9UCR0; Q9UCR1; Q9UCR2; Q9UCR3; Q9UCR4; A8UHA1; E9PHP7
• Molecular Weight: 863
• NCBI Official Full Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
• NCBI Official Synonym Full Names: ectonucleotide pyrophosphatase/phosphodiesterase 2
• NCBI Official Symbol: ENPP2
• NCBI Official Synonym Symbols: ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
• NCBI Protein Information: ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; autotaxin-t; plasma lysophospholipase D; extracellular lysophospholipase D; phosphodiesterase I/nucleotide pyrophosphatase 2
• UniProt Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
• Protein Family: Ectonucleotide pyrophosphatase/phosphodiesterase family
• UniProt Gene Name: ENPP2
• UniProt Synonym Gene Names: ATX; PDNP2; E-NPP 2; LysoPLD
• UniProt Entry Name: ENPP2_HUMAN

NCBI Description

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008]

Uniprot Description

ENPP2: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - ether lipid; Phosphodiesterase; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; EC 3.1.4.39

Chromosomal Location of Human Ortholog: 8q24.1

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: nucleotide diphosphatase activity; phosphodiesterase I activity; nucleic acid binding; hydrolase activity; zinc ion binding; calcium ion binding; transcription factor binding; scavenger receptor activity; alkylglycerophosphoethanolamine phosphodiesterase activity; polysaccharide binding; lysophospholipase activity

Biological Process: G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; phospholipid catabolic process; immune response; cell motility; chemotaxis; regulation of angiogenesis; phosphate metabolic process; regulation of cell migration