Rabbit anti-Human ENPEP / Aminopeptidase A Polyclonal Antibody | anti-ENPEP antibody

Rabbit Polyclonal to Human ENPEP / Aminopeptidase A

Cat. Num. AAA244344

• Gene Names: ENPEP; APA; CD249; gp160
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: ENPEP / Aminopeptidase A; Polyclonal Antibody; Rabbit Polyclonal to Human ENPEP / Aminopeptidase A; Anti-ENPEP / Aminopeptidase A Antibody IHC-plus; ENPEP; APA; Aminopeptidase A; AP-A; CD249 antigen; Differentiation antigen gp160; EAP; Glutamyl aminopeptidase; gp160; CD249; Human ENPEP; anti-ENPEP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human Glutamyl aminopeptidase. Predicted cross-reactivity based on amino acid sequence homology: mouse (85%), rat (86%), bovine (89%), pig (90%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.1 M Tris-glycine, pH 7, 10% Glycerol, 0.01% Thimerosal
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 957

• Applicable Applications for anti-ENPEP antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:500 - 1:3000)
• Target Species: Human
• Immunogen Description: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 689 and 932 of Human ENPEP.
• Immunogen Type: Recombinant protein
• Immunogen: ENPEP / Aminopeptidase A antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 689 and 932 of Human ENPEP.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-ENPEP antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ENPEP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Sample(30 ug whole cell lysate). A: Hep G2. B: HeLa S3. 7.5% SDS PAGE. ENPEP antibody diluted at 1:1000.)

Related Product Information for anti-ENPEP antibody

Human aminopeptidase A is a cell surface differentiation-related glycoprotein of 160 kD expressed by epithelial cells of the glomerulus and proximal tubules of the human kidney and in the majority of renal cell carcinomas. Expression of aminopeptidase A by cultured renal cell carcinomas correlated with the resistance of these cells to the antiproliferative effects of alpha interferon.

Product Categories/Family for anti-ENPEP antibody

Family: Exopeptidase; Subfamily: Metallopeptidase M1

NCBI and Uniprot Product Information

• NCBI GI #: 132814467
• NCBI GeneID: 2028
• NCBI Accession #: NP_001968.3
• NCBI GenBank Nucleotide #: NM_001977.3
• UniProt Accession #: Q07075; Q504U2
• Molecular Weight: 109,244 Da
• NCBI Official Full Name: glutamyl aminopeptidase
• NCBI Official Synonym Full Names: glutamyl aminopeptidase (aminopeptidase A)
• NCBI Official Symbol: ENPEP
• NCBI Official Synonym Symbols: APA; CD249; gp160
• NCBI Protein Information: glutamyl aminopeptidase; EAP; AP-A; aminopeptidase A; differentiation antigen gp160
• UniProt Protein Name: Glutamyl aminopeptidase
• UniProt Gene Name: ENPEP
• UniProt Synonym Gene Names: EAP; AP-A
• UniProt Entry Name: AMPE_HUMAN

Uniprot Description

ENPEP: Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells. Belongs to the peptidase M1 family.

Protein type: Membrane protein, integral; EC 3.4.11.7; Protease

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: integral to plasma membrane; lysosomal membrane; apical part of cell; apical plasma membrane; plasma membrane; cytoplasmic vesicle; brush border; external side of plasma membrane

Molecular Function: metallopeptidase activity; zinc ion binding; aminopeptidase activity

Biological Process: cell proliferation; cell migration; cellular protein metabolic process; cell-cell signaling; angiotensin catabolic process in blood; angiogenesis; angiotensin maturation; proteolysis; glomerulus development; regulation of systemic arterial blood pressure by renin-angiotensin