Western Blot (WB)
(ENOPH1 rabbit polyclonal antibody. Western Blot analysis of ENOPH1 expression in human colon.)
Rabbit anti-Human, Mouse ENOPH1 Polyclonal Antibody | anti-ENOPH1 antibody
ENOPH1 (Enolase-phosphatase E1, 2,3-diketo-5-methylthio-1-phosphopentane Phosphatase, MASA Homolog, MASA, MSTP145, DKFZp586M0524, E1, FLJ12594) (AP)
Gene Names
ENOPH1; E1; MASA; mtnC; MST145
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ENOPH1; Polyclonal Antibody; ENOPH1 (Enolase-phosphatase E1; 2; 3-diketo-5-methylthio-1-phosphopentane Phosphatase; MASA Homolog; MASA; MSTP145; DKFZp586M0524; E1; FLJ12594) (AP); anti-ENOPH1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ENOPH1. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-ENOPH1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human ENOPH1, aa1-261 (NP_067027.1).
Immunogen Sequence
MVVLSVPAEVTVILLDIEGTTTPIAFVKDILFPYIEENVKEYLQTHWEEEECQQDVSLLRKQAEEDAHLDGAVPIPAASGNGVDDLQQMIQAVVDNVCWQMSLDRKTTALKQLQGHMWRAAFTAGRMKAEFFADVVPAVRKWREAGMKVYIYSSGSVEAQKLLFGHSTEGDILELVDGHFDTKIGHKVESESYRKIADSIGCSTNNILFLTDVTREASAAEEADVHVAVVVRPGNAGLTDDEKTYYSLITSFSELYLPSST
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(ENOPH1 rabbit polyclonal antibody. Western Blot analysis of ENOPH1 expression in human colon.)
Western Blot (WB)
(ENOPH1 rabbit polyclonal antibody. Western Blot analysis of ENOPH1 expression in human colon.)
Western Blot (WB)
(ENOPH1 rabbit polyclonal antibody. Western Blot analysis of ENOPH1 expression in mouse spleen.)
Western Blot (WB)
(ENOPH1 rabbit polyclonal antibody. Western Blot analysis of ENOPH1 expression in mouse spleen.)
Western Blot (WB)
(Western Blot analysis of ENOPH1 expression in transfected 293T cell line by ENOPH1 polyclonal antibody. Lane 1: ENOPH1 transfected lysate (28.9kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of ENOPH1 expression in transfected 293T cell line by ENOPH1 polyclonal antibody. Lane 1: ENOPH1 transfected lysate (28.9kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-ENOPH1 antibody
Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
Product Categories/Family for anti-ENOPH1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
enolase-phosphatase E1 isoform 1
NCBI Official Synonym Full Names
enolase-phosphatase 1
NCBI Official Symbol
ENOPH1
NCBI Official Synonym Symbols
E1; MASA; mtnC; MST145
NCBI Protein Information
enolase-phosphatase E1
UniProt Protein Name
Enolase-phosphatase E1
UniProt Gene Name
ENOPH1
UniProt Entry Name
ENOPH_HUMAN
Uniprot Description
ENOPH1: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK- MTPene). Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Phosphatase (non-protein); Amino Acid Metabolism - cysteine and methionine; EC 3.1.3.77
Chromosomal Location of Human Ortholog: 4q21.22
Cellular Component: cytosol; nucleus
Molecular Function: acireductone synthase activity; magnesium ion binding
Biological Process: dephosphorylation; methionine salvage; sulfur amino acid metabolic process; polyamine metabolic process
Research Articles on ENOPH1
Similar Products
Product Notes
The ENOPH1 enoph1 (Catalog #AAA6377393) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENOPH1 (Enolase-phosphatase E1, 2,3-diketo-5-methylthio-1-phosphopentane Phosphatase, MASA Homolog, MASA, MSTP145, DKFZp586M0524, E1, FLJ12594) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ENOPH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ENOPH1 enoph1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENOPH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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