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Western Blot (WB)
(Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse NSE Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Rabbit anti-Mouse Enolase Polyclonal Antibody | anti-NSE antibody
Polyclonal Antibody to Enolase, Neuron Specific (NSE)
Gene Names
Eno2; NSE; Eno-2; AI837106; D6Ertd375e
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Enolase; Polyclonal Antibody; Polyclonal Antibody to Enolase; Neuron Specific (NSE); anti-NSE antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NSE. It has beenselected for its ability to recognize NSE in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
432
Applicable Applications for anti-NSE antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
NSE (Ser2~Leu434)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Human, Mouse, Rat
Conjugate
No Conjugate
Immunogen
Recombinant NSE (Ser2~Leu434) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043582
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse NSE Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse NSE Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate;;Lane 2: NSE knockout HepG2 cell lysate;;Predicted MW: 47kDa ;Observed MW: 50kDa;Primary Ab: 2ug/ml Rabbit Anti-Mouse NSE Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate;;Lane 2: NSE knockout HepG2 cell lysate;;Predicted MW: 47kDa ;Observed MW: 50kDa;Primary Ab: 2ug/ml Rabbit Anti-Mouse NSE Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Product Categories/Family for anti-NSE antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,297 Da
NCBI Official Full Name
gamma-enolase isoform 1
NCBI Official Synonym Full Names
enolase 2, gamma neuronal
NCBI Official Symbol
Eno2
NCBI Official Synonym Symbols
NSE; Eno-2; AI837106; D6Ertd375e
NCBI Protein Information
gamma-enolase
UniProt Protein Name
Enolase 2, gamma neuronal, isoform CRA_a
Protein Family
UniProt Gene Name
Eno2
Research Articles on NSE
Similar Products
Product Notes
The NSE eno2 (Catalog #AAA2028314) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Enolase, Neuron Specific (NSE) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Enolase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the NSE eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Enolase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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