Sheep anti-Human, Mouse ENDOTHELIN B RECEPTOR Polyclonal Antibody | anti-Ednrb antibody

SHEEP ANTI RAT ENDOTHELIN B RECEPTOR (C-TERMINAL)

Cat. Num. AAA223880

• Gene Names: Ednrb; Etb; Ednra
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Immunoprecipitation, Immunohistochemistry
• Synonyms: ENDOTHELIN B RECEPTOR; Polyclonal Antibody; SHEEP ANTI RAT ENDOTHELIN B RECEPTOR (C-TERMINAL); anti-Ednrb antibody

• Host: Sheep
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a non-specific receptor for endothelin 1, 2, and 3, and mediates its action by G protein association and activation of a phosphatidylinositol-calcium second messenger system. This item recognizes epitopes on the cytoplasmic side of the receptor and therefore the cell membrane must be permeable to the antibody. This item does not block the binding of endothelins. This item is reported as suitable for use in immunocytochemistry on acetone fixed cells.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.41mg/ml (varies by lot)
• Sequence Length: 442

• Applicable Applications for anti-Ednrb antibody: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin
• Application Notes: Immunofluorescence: Minimum Dilution: 10; Maximum Dilution: 20ug/ml;; Immunoprecipitation: Minimum Dilution: 20; Maximum Dilution: 40ug/ml;; Immunohistology - Paraffin: Minimum Dilution: 10; Maximum Dilution: 20ug/ml
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Keyhole limpet haemocyanin (KLH) conjugated C-terminal peptide KANDHGYDNFRSSNN corresponding to amino acids 424 to 437 of rat endothelin B receptor.; Buffer Solution
• Antiserum Preparation: Antiserum to rat ET-B (CT) was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-Ednrb antibody

MBS223880 specifically recognises an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a non-specific receptor for endothelin 1, 2, and 3, and mediates its action by G protein association and activation of a phosphatidylinositol-calcium second messenger system. MBS223880 recognizes epitopes on the cytoplasmic side of the receptor and therefore the cell membrane must be permeable to the antibody. MBS223880 does not block the binding of endothelins. MBS223880 is reported as suitable for use in immunocytochemistry on acetone fixed cells.

NCBI and Uniprot Product Information

• NCBI GI #: 8393333
• NCBI GeneID: 50672
• NCBI Accession #: NP_059029.1
• NCBI GenBank Nucleotide #: NM_017333.1
• Molecular Weight: 49,455 Da
• NCBI Official Full Name: endothelin B receptor
• NCBI Official Synonym Full Names: endothelin receptor type B
• NCBI Official Symbol: Ednrb
• NCBI Official Synonym Symbols: Etb; Ednra
• NCBI Protein Information: endothelin B receptor; ET-B; ET-BR; endothelin receptor non-selective type; endothelin-B receptor
• UniProt Protein Name: Endothelin B receptor
• Protein Family: Endothelin B receptor
• UniProt Gene Name: Ednrb
• UniProt Synonym Gene Names: ET-B; ET-BR
• UniProt Entry Name: EDNRB_RAT

Uniprot Description

ETB: a G protein-coupled receptor which activates a phosphatidylinoitol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutation in endothelin receptor type B gene. Essential component in the normal development of two neuronal crest-derived cell lineages. Two splice variant isoforms have been described.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral

Cellular Component: nuclear membrane; membrane; integral to membrane; plasma membrane; lipid raft

Molecular Function: endothelin receptor activity; G-protein coupled receptor activity; peptide hormone binding; receptor activity; type 1 angiotensin receptor binding

Biological Process: epithelial fluid transport; cGMP-mediated signaling; pigmentation during development; regulation of fever; negative regulation of cellular protein metabolic process; response to lipopolysaccharide; response to pain; negative regulation of transcription from RNA polymerase II promoter; sensory perception of pain; vein smooth muscle contraction; vasodilation; enteric nervous system development; response to organic cyclic substance; regulation of epithelial cell proliferation; elevation of cytosolic calcium ion concentration; regulation of blood pressure; positive regulation of cell proliferation; melanocyte differentiation; regulation of pH; neural crest cell migration; aging; vasoconstriction; posterior midgut development; macrophage chemotaxis; peripheral nervous system development; G-protein coupled receptor protein signaling pathway; negative regulation of neuron maturation; pigmentation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of protein amino acid phosphorylation; regulation of sensory perception of pain; negative regulation of apoptosis