Rabbit anti-Human ELOVL2 Polyclonal Antibody | anti-ELOVL2 antibody

ELOVL2, NT (ELOVL2, SSC2, Elongation of very long chain fatty acids protein 2, 3-keto acyl-CoA synthase ELOVL2, ELOVL fatty acid elongase 2)

Cat. Num. AAA642149

• Gene Names: ELOVL2; SSC2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ELOVL2; Polyclonal Antibody; NT (ELOVL2; SSC2; Elongation of very long chain fatty acids protein 2; 3-keto acyl-CoA synthase ELOVL2; ELOVL fatty acid elongase 2); Anti -ELOVL2; anti-ELOVL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ELOVL2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: ELOVL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7~36 amino acids from the N-terminal region of human ELOVL2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ELOVL2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). ELOVL2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with ELOVL2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ELOVL2 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ELOVL2 antibody

ELOVL2 could be implicated in tissue-specific synthesis of very long chain fatty acids and sphingolipids. This protein may catalyze one or both of the reduction reaction in fatty acid elongation, i.e., conversion of beta-ketoacyl CoA to beta-hydroxyacyl CoA or reduction of trans-2-enoyl CoA to the saturated acyl CoA derivative (By similarity).

Product Categories/Family for anti-ELOVL2 antibody

Antibodies; Abs to Enzymes; Synthase

NCBI and Uniprot Product Information

• NCBI GI #: 157388945
• NCBI GeneID: 54898
• NCBI Accession #: NP_060240.3
• NCBI GenBank Nucleotide #: NM_017770.3
• UniProt Accession #: Q9NXB9; Q6P9E1; Q86W94
• Molecular Weight: 34,585 Da
• NCBI Official Full Name: elongation of very long chain fatty acids protein 2
• NCBI Official Synonym Full Names: ELOVL fatty acid elongase 2
• NCBI Official Symbol: ELOVL2
• NCBI Official Synonym Symbols: SSC2
• NCBI Protein Information: elongation of very long chain fatty acids protein 2; ELOVL FA elongase 2; 3-keto acyl-CoA synthase ELOVL2; very-long-chain 3-oxoacyl-CoA synthase 2; elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 2
• UniProt Protein Name: Elongation of very long chain fatty acids protein 2
• Protein Family: Elongation of very long chain fatty acids protein
• UniProt Gene Name: ELOVL2
• UniProt Synonym Gene Names: SSC2; ELOVL FA elongase 2
• UniProt Entry Name: ELOV2_HUMAN

Uniprot Description

ELOVL2: Condensing enzyme that catalyzes the synthesis of polyunsaturated very long chain fatty acid (C20- and C22-PUFA). Acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C20:4(n-6) acyl-CoA. Belongs to the ELO family.

Protein type: Membrane protein, multi-pass; Lipid Metabolism - unsaturated fatty acid biosynthesis; EC 2.3.1.199; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p24.2

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; fatty acid elongase activity

Biological Process: unsaturated fatty acid metabolic process; very-long-chain fatty acid biosynthetic process; linoleic acid metabolic process; triacylglycerol biosynthetic process; unsaturated fatty acid biosynthetic process; cellular lipid metabolic process