Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Rat ELOA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Elongin A (ELOA) Polyclonal Antibody | anti-ELOA antibody
Polyclonal Antibody to Elongin A (ELOA)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- Q EPIVSHPKPG KVHSNTFQDR LGVSHLGEHQ GKGAVSQNKP HKSSHKEKRP VDARGDEKSS VMGREKSHKA SSKEESRRLL SEDSAKEKLP SSVVKKEKDR EGNSLKKKLS PALDVASDNH FKKPKHKDSE
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Rat ELOA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Rat ELOA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Skeletal muscle Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Skeletal muscle Tissue))
NCBI and Uniprot Product Information
NCBI Description
subunit A of the transcription factor B (SIII) complex (also known as elongin); frees RNA Polymerase II from transient pausing at template-encoded arresting sites [RGD, Feb 2006]
Uniprot Description
TCEB3: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). Heterotrimer of an A (A1, A2 or A3), B and C subunit. The C subunit mediates the binding of the elongin BC complex to subunit A.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 5q36
Cellular Component: extracellular space; integral component of membrane; nucleus; transcription elongation factor complex
Molecular Function: double-stranded DNA binding; translation elongation factor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; transcription elongation from RNA polymerase II promoter; translational elongation
Research Articles on ELOA
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Product Notes
The ELOA eloa (Catalog #AAA2013790) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Elongin A (ELOA) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Elongin A (ELOA) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ELOA eloa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- Q EPIVSHPKPG KVHSNTFQDR LGVSHLGEHQ GKGAVSQNKP HKSSHKEKRP VDARGDEKSS VMGREKSHKA SSKEESRRLL SEDSAKEKLP SSVVKKEKDR EGNSLKKKLS PALDVASDNH FKKPKHKDSE. It is sometimes possible for the material contained within the vial of "Elongin A (ELOA), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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