Western Blot (WB)
(Western blot analysis of ELF5 expression in HeLa (A), HuvEc (B), Jurkat (C) whole cell lysates.)
Rabbit ELF5 Polyclonal Antibody | anti-ELF5 antibody
Anti-ELF5 Antibody
Western Blot (WB)
(Western blot analysis of ELF5 expression in HeLa (A), HuvEc (B), Jurkat (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of ELF5 expression in HeLa (A), HuvEc (B), Jurkat (C) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of ELF5 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of ELF5 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]
Uniprot Description
ELF5: Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation. Belongs to the ETS family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 11p13-p12
Cellular Component: cytoplasm; nucleus
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; cell proliferation; ectoderm development; positive regulation of transcription from RNA polymerase II promoter; cell differentiation
Research Articles on ELF5
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Product Notes
The ELF5 elf5 (Catalog #AAA820369) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ELF5 Antibody reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's ELF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC), Immunoprecipitation (IP). WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500), IP (1/10 - 1/100). Researchers should empirically determine the suitability of the ELF5 elf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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