Rabbit anti-Human ELF4 Polyclonal Antibody | anti-ELF4 antibody

ELF4, NT (ELF4, ELFR, MEF, ETS-related transcription factor Elf-4, E74-like factor 4, Myeloid Elf-1-like factor) (PE)

Cat. Num. AAA6295225

• Gene Names: ELF4; MEF; ELFR
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ELF4; Polyclonal Antibody; NT (ELF4; ELFR; MEF; ETS-related transcription factor Elf-4; E74-like factor 4; Myeloid Elf-1-like factor) (PE); Myeloid Elf-1-like factor; anti-ELF4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ELF4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ELF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-196 amino acids from the N-terminal region of human ELF4.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ELF4 (arrow) using rabbit polyclonal ELF4 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ELF4 gene.)

Related Product Information for anti-ELF4 antibody

The protein encoded by this gene is a transcriptional activator that binds and activates the promoters of the CSF2, IL3, IL8, and PRF1 genes. The encoded protein is involved in natural killer cell development and function, innate immunity, and induction of cell cycle arrest in naive CD8+ cells. Two transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-ELF4 antibody

Antibodies; Transcription Factors

References

Yamada, T., et al. Nat. Immunol. 10(6):618-626(2009). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Moore, S.D., et al. Leuk. Res. 30(8):1037-1042(2006). Liu, Y., et al. Mol. Cell. Biol. 26(8):3114-3123(2006). Koga, T., et al. FEBS Lett. 579(13):2811-2816(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4503555
• NCBI GeneID: 2000
• NCBI Accession #: NP_001412.1; NP_001120669.1
• NCBI GenBank Nucleotide #: NM_001421.3
• UniProt Accession #: Q99607; O60435; D3DTG1
• Molecular Weight: 70,730 Da
• NCBI Official Full Name: ETS-related transcription factor Elf-4
• NCBI Official Synonym Full Names: E74-like factor 4 (ets domain transcription factor)
• NCBI Official Symbol: ELF4
• NCBI Official Synonym Symbols: MEF; ELFR
• NCBI Protein Information: ETS-related transcription factor Elf-4; ELF4; myeloid Elf-1-like factor
• UniProt Protein Name: ETS-related transcription factor Elf-4
• Protein Family: ETS-related transcription factor
• UniProt Gene Name: ELF4
• UniProt Synonym Gene Names: ELFR; MEF
• UniProt Entry Name: ELF4_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional activator that binds and activates the promoters of the CSF2, IL3, IL8, and PRF1 genes. The encoded protein is involved in natural killer cell development and function, innate immunity, and induction of cell cycle arrest in naive CD8+ cells. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]

Uniprot Description

ELF4: Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. Acts synergistically with RUNX1 to transactivate the IL3 promoter. Also transactivates the PRF1 promoter in natural killer (NK) cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2. Controls cell senescence in a p53-dependent manner. Can also promote cellular transformation through inhibition of the p16 pathway. A chromosomal aberration involving ELF4 has been found in a case of acute myeloid leukemia (AML). Translocation t(X;21)(q25-26;q22) with ERG. Belongs to the ETS family.

Protein type: Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xq26

Cellular Component: nucleoplasm; PML body

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; natural killer cell proliferation; NK T cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; cell differentiation