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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human Elastin Microfibril Interface Located Protein 1 Polyclonal Antibody | anti-EMILIN1 antibody

Polyclonal Antibody to Elastin Microfibril Interface Located Protein 1 (EMILIN1)

Gene Names
EMILIN1; EMI; gp115; EMILIN
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Elastin Microfibril Interface Located Protein 1; Polyclonal Antibody; Polyclonal Antibody to Elastin Microfibril Interface Located Protein 1 (EMILIN1); anti-EMILIN1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against EMILIN1. It has beenselected for its ability to recognize EMILIN1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QLSLK DLTGPAGEAG PPGPPGLQGP PGPAGPPGSP GKDGQEGPIG PPGPQGEQGV EGAPAAPVPQ VAFSAALSLP RSEPGTVPFD RVLLNDGGYY DPETGVFTAP LAGRYLLSAV LTGHRHEKVE AVLSRSNQGV ARVDSGGYEP EGLENKPVAE SQPSPGTLGV FSLILPLQAG DTVCVDLVMG
Sequence Length
1016
Applicable Applications for anti-EMILIN1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
EMILIN1 (Gln806~Ala1016)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant EMILIN1 (Gln806~Ala1016) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2042141
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Intestine tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Intestine tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,928 Da
NCBI Official Full Name
EMILIN-1
NCBI Official Synonym Full Names
elastin microfibril interfacer 1
NCBI Official Symbol
EMILIN1
NCBI Official Synonym Symbols
EMI; gp115; EMILIN
NCBI Protein Information
EMILIN-1
UniProt Protein Name
EMILIN-1
Protein Family
UniProt Gene Name
EMILIN1
UniProt Synonym Gene Names
EMI; Elastin microfibril interfacer 1

NCBI Description

This gene encodes an extracellular matrix glycoprotein that is characterized by an N-terminal microfibril interface domain, a coiled-coiled alpha-helical domain, a collagenous domain and a C-terminal globular C1q domain. The encoded protein associates with elastic fibers at the interface between elastin and microfibrils and may play a role in the development of elastic tissues including large blood vessels, dermis, heart and lung. [provided by RefSeq, Sep 2009]

Uniprot Description

EMILIN1: May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.

Protein type: Cell adhesion; Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: collagen; extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: extracellular matrix constituent conferring elasticity; identical protein binding; protein binding

Biological Process: cell adhesion; cell migration; cell-matrix adhesion

Research Articles on EMILIN1

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Product Notes

The EMILIN1 emilin1 (Catalog #AAA2026449) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Elastin Microfibril Interface Located Protein 1 (EMILIN1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Elastin Microfibril Interface Located Protein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the EMILIN1 emilin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QLSLK DLTGPAGEAG PPGPPGLQGP PGPAGPPGSP GKDGQEGPIG PPGPQGEQGV EGAPAAPVPQ VAFSAALSLP RSEPGTVPFD RVLLNDGGYY DPETGVFTAP LAGRYLLSAV LTGHRHEKVE AVLSRSNQGV ARVDSGGYEP EGLENKPVAE SQPSPGTLGV FSLILPLQAG DTVCVDLVMG. It is sometimes possible for the material contained within the vial of "Elastin Microfibril Interface Located Protein 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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