Rabbit anti-Human Elastin, Aortic Polyclonal Antibody | anti-ELN antibody

Elastin, Aortic

Cat. Num. AAA623261

• Gene Names: ELN; WS; WBS; SVAS
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Elastin; Aortic; Polyclonal Antibody; Anti -Elastin; anti-ELN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human insoluble elastin and human tropoelastin. Low Species Crossreactivity: bovine, rabbit, canine. This antibody shows little if any reactivity with rat elastin.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder. Reconstitute with sterile 40-50% glycerol, dH2O, 0.01% Thimerosal.

• Applicable Applications for anti-ELN antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.; Dilution: ELISA: 1:1000 -1:2000; Western Blot: 1:200 -1:500; Immunoprecipitation: 1:100 -1:200; Immunohistochemistry: 1:50 -1:100; In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.
• Immunogen: Insoluble elastin purified from human aorta by hot sodium hydroxide.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ELN antibody

Elastin is a protein in connective tissue that is elastic and allows many tissues in the body to resume their shape after stretching or contracting. Elastin helps skin to return to its original position when it is poked or pinched. Elastin is also an important load-bearing tissue in the bodies of mammals and used in places where mechanical energy is required to be stored. Elastin is particularly abundant in large elastic blood vessels such as the aorta. It is also very important in the lungs, elastic ligaments, the skin, the bladder, and elastic cartilage.

Product Categories/Family for anti-ELN antibody

Antibodies; Abs to Elastin

NCBI and Uniprot Product Information

• NCBI GI #: 525313599
• NCBI GeneID: 2006
• NCBI Accession #: NP_001265868.1
• NCBI GenBank Nucleotide #: NM_001278939.1
• UniProt Accession #: P15502; O15336; O15337; Q14233; Q14234; Q14235; Q14238; Q6P0L4; Q6ZWJ6; Q75MU5; Q7Z316; B3KTS6
• Molecular Weight: 68,469 Da
• NCBI Official Full Name: elastin isoform m
• NCBI Official Synonym Full Names: elastin
• NCBI Official Symbol: ELN
• NCBI Official Synonym Symbols: WS; WBS; SVAS
• NCBI Protein Information: elastin; tropoelastin
• UniProt Protein Name: Elastin
• Protein Family: Elastin
• UniProt Gene Name: ELN
• UniProt Entry Name: ELN_HUMAN

NCBI Description

This gene encodes a protein that is one of the two components of elastic fibers. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

elastin: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle. Defects in ELN are the cause of cutis laxa, autosomal dominant, type 1 (ADCL1). A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. Additional variable clinical features are gastrointestinal diverticula, hernia, and genital prolapse. Rare manifestations are pulmonary artery stenosis, aortic aneurysm, bronchiectasis, and emphysema. Defects in ELN are the cause of supravalvular aortic stenosis (SVAS). SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome. ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. Belongs to the elastin family. 13 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: protein binding; extracellular matrix structural constituent

Biological Process: extracellular matrix disassembly; cell proliferation; organ morphogenesis; extracellular matrix organization and biogenesis; blood circulation; respiratory gaseous exchange

Disease: Cutis Laxa, Autosomal Dominant 1; Williams-beuren Syndrome; Supravalvular Aortic Stenosis