Rabbit anti-Human Elastase 2 (ELA2) Polyclonal Antibody | anti-ELA2 antibody
PE-Linked Polyclonal Antibody to Elastase 2, Neutrophil (ELA2)
Gene Names
CELA2A; PE-1; ELA2A
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Antigen-specific Affinity Chromatography.
Synonyms
Elastase 2 (ELA2); Polyclonal Antibody; PE-Linked Polyclonal Antibody to Elastase 2; Neutrophil (ELA2); ELANE; HLE; HNE; NE; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Human Leukocyte Elastase; PMN Elastase; anti-ELA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen-specific Affinity Chromatography.
Form/Format
Supplied as solution form in 10 mM Tris, 150 mM NaCl, pH 8.2 50% glycerol 0.05% Proclin-300.
Concentration
0.12mg/ml (varies by lot)
Applicable Applications for anti-ELA2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunofluorescence (IF).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Homo sapiens (Human)
Source
Antibody labeling
Cross Reactivity
Human
Label
PE
Unconjugated Antibody
MBS2027220
Traits
Liquid
Immunogen
Recombinant NE (Ile30~Gln247) expressed in E.coli ( MBS2029953).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
elastase 2
NCBI Official Synonym Full Names
chymotrypsin like elastase family member 2A
NCBI Official Symbol
CELA2A
NCBI Official Synonym Symbols
PE-1; ELA2A
NCBI Protein Information
chymotrypsin-like elastase family member 2A
UniProt Protein Name
Chymotrypsin-like elastase family member 2A
UniProt Gene Name
CELA2A
UniProt Synonym Gene Names
ELA2A
NCBI Description
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. [provided by RefSeq, May 2009]
Uniprot Description
CELA2A: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily.
Protein type: EC 3.4.21.71; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p36.21
Cellular Component: extracellular space
Molecular Function: serine hydrolase activity
Research Articles on ELA2
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Product Notes
The ELA2 cela2a (Catalog #AAA2039236) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PE-Linked Polyclonal Antibody to Elastase 2, Neutrophil (ELA2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Elastase 2 (ELA2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunofluorescence (IF). . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ELA2 cela2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Elastase 2 (ELA2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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