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Western Blot (WB) (Western Blot analysis of EIF5B polyclonal antibody)

Rabbit anti-Human EIF5B Polyclonal Antibody | anti-EIF5B antibody

EIF5B Polyclonal Antibody

Gene Names
EIF5B; IF2
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
EIF5B; Polyclonal Antibody; EIF5B Polyclonal Antibody; Eukaryotic translation initiation factor 5B; eIF-5B; Translation initiation factor IF-2; IF2; KIAA0741; anti-EIF5B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
EIF5B polyclonal antibody detects endogenous levels of EIF5B protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1220
Applicable Applications for anti-EIF5B antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant full length Human EIF5B.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of EIF5B polyclonal antibody)

Western Blot (WB) (Western Blot analysis of EIF5B polyclonal antibody)
Related Product Information for anti-EIF5B antibody
In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors. The eukaryotic translation initiation factor 5 (eIF5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal subunit, a function necessary for the subsequent joining of the 40S and 60S subunits to form the 80S initiation complex. eIF5B (Eukaryotic translation initiation factor 5B), also known as Translation initiation factor IF-2, is a 1, 120 amino acid cytoplasmic protein that functions in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. eIF5B interacts with Annexin V, an anticoagulant protein, in a calcium and phospholipid-dependent manner. Since eIF5B is conserved among all three kingdoms of life, it is also known as an universal initiation factor.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 179kDa
NCBI Official Full Name
eukaryotic translation initiation factor 5B
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 5B
NCBI Official Symbol
EIF5B
NCBI Official Synonym Symbols
IF2
NCBI Protein Information
eukaryotic translation initiation factor 5B
UniProt Protein Name
Eukaryotic translation initiation factor 5B
UniProt Gene Name
EIF5B
UniProt Synonym Gene Names
IF2; KIAA0741; eIF-5B
UniProt Entry Name
IF2P_HUMAN

NCBI Description

Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008]

Uniprot Description

EIF5B: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2. Belongs to the IF-2 family.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding; translation initiation factor activity

Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; regulation of translational initiation

Research Articles on EIF5B

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Product Notes

The EIF5B eif5b (Catalog #AAA3007820) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF5B Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF5B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the EIF5B eif5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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