Goat EIF5A Polyclonal Antibody | anti-EIF5A antibody

Goat Polyclonal to Human EIF5A

Cat. Num. AAA246749

• Gene Names: EIF5A; EIF-5A; EIF5A1; eIF5AI
• Reactivity: Gorilla, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: EIF5A; Polyclonal Antibody; Goat Polyclonal to Human EIF5A; Anti-EIF5A Antibody (N-Terminus) IHC-plus; EIF-4D; EIF-5A; EIF-5A-1; EIF-5A1; EIF5A1; Initiation factor 5a; Rev-binding factor; EIF5AI; Human EIF5A; anti-EIF5A antibody

• Host: Goat
• Reactivity: Gorilla, Human
• Clonality: Polyclonal
• Specificity: Human EIF5A. Reported variants NP_001137233.1, NP_001961.1 and NP_001137234.1 represent identical protein:
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 154

• Applicable Applications for anti-EIF5A antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (3.75 ug/ml), WB (1 - 3 ug/ml); Usage: Western Blot: Approx 20kD band observed in MOLT4 lysates (calculated MW of 20.2kD according to NP_001137232.1). Recommended concentration: 1-3 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide C-RPPHRASFLKRLESK from an internal region (near N-terminus) of human EIF5A (NP_001137232.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Monkey, Dog (87%).
• Immunogen Type: Synthetic peptide
• Immunogen: EIF5A antibody was raised against synthetic peptide C-RPPHRASFLKRLESK from an internal region (near N-terminus) of human EIF5A (NP_001137232.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Monkey, Dog (87%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-EIF5A antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-EIF5A antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-EIF5A antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(EIF5A antibody (0.1 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503545
• NCBI GeneID: 1984
• NCBI Accession #: NP_001961.1
• NCBI GenBank Nucleotide #: NM_001970.4
• UniProt Accession #: P63241; P10159; Q16182; Q7L7L3; Q7Z4L1; Q9D0G2; A8K9A0; D3DTP2; AAB29229
• Molecular Weight: 20,170 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 5A-1 isoform B
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 5A
• NCBI Official Symbol: EIF5A
• NCBI Official Synonym Symbols: EIF-5A; EIF5A1; eIF5AI
• NCBI Protein Information: eukaryotic translation initiation factor 5A-1; eIF-4D; eIF-5A1; eIF-5A-1; rev-binding factor; eukaryotic initiation factor 5A
• UniProt Protein Name: Eukaryotic translation initiation factor 5A-1
• Protein Family: Translation initiation factor
• UniProt Gene Name: EIF5A
• UniProt Synonym Gene Names: eIF-5A-1; eIF-5A1; eIF-5A
• UniProt Entry Name: IF5A1_HUMAN

Uniprot Description

EIF5A: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. Belongs to the eIF-5A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 17p13-p12

Cellular Component: endoplasmic reticulum membrane; membrane; cytoplasm; nuclear pore; nucleus; cytosol; annulate lamellae

Molecular Function: protein binding; RNA binding; ribosome binding; protein N-terminus binding; translation elongation factor activity; U6 snRNA binding

Biological Process: mRNA export from nucleus; cellular protein metabolic process; translational frameshifting; apoptosis; positive regulation of cell proliferation; hypusine biosynthetic process from peptidyl-lysine; nucleocytoplasmic transport; positive regulation of translational elongation; protein export from nucleus; post-translational protein modification; positive regulation of translational termination