Rabbit anti-Human EIF2C1 Polyclonal Antibody | anti-EIF2C1 antibody

EIF2C1, NT (Protein Argonaute-1, Argonaute1, hAgo1, Eukaryotic Translation Initiation Factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding Protein Q99, AGO1) (AP)

Cat. Num. AAA6473673

• Gene Names: AGO1; Q99; EIF2C; hAgo1; EIF2C1; GERP95
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: EIF2C1; Polyclonal Antibody; NT (Protein Argonaute-1; Argonaute1; hAgo1; Eukaryotic Translation Initiation Factor 2C 1; eIF-2C 1; eIF2C 1; Putative RNA-binding Protein Q99; AGO1) (AP); anti-EIF2C1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AGO1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-EIF2C1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human AGO1 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AGO1 Antibody (N-term) in K562 cell line lysates (35ug/lane). AGO1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(AGO1 Antibody (N-term) (RB18965) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AGO1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-EIF2C1 antibody

Antibodies; Translation Initiation Factors

References

1. Weinmann, L., Cell 136(3), 496-507 (2009). 2. Horikawa, Y., Clin. Cancer Res. 14(23), 7956-7962 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 6912352
• NCBI GeneID: 26523
• NCBI Accession #: NP_036331
• NCBI GenBank Nucleotide #: NM_012199
• UniProt Accession #: Q9UL18
• Molecular Weight: 97kDa
• NCBI Official Full Name: protein argonaute-1 isoform 1
• NCBI Official Synonym Full Names: argonaute RISC component 1
• NCBI Official Symbol: AGO1
• NCBI Official Synonym Symbols: Q99; EIF2C; hAgo1; EIF2C1; GERP95
• NCBI Protein Information: protein argonaute-1
• UniProt Protein Name: Protein argonaute-1
• UniProt Gene Name: AGO1
• UniProt Synonym Gene Names: EIF2C1; Argonaute1; hAgo1; eIF-2C 1; eIF2C 1
• UniProt Entry Name: AGO1_HUMAN

NCBI Description

This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]

Uniprot Description

AGO1: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, EIF2C2/AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with LIMD1, WTIP and AJUBA. Belongs to the argonaute family. Ago subfamily.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; polysome; cytoplasm; cytosol; ribonucleoprotein complex

Molecular Function: endoribonuclease activity; protein binding; miRNA binding; RNA binding

Biological Process: epidermal growth factor receptor signaling pathway; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; innate immune response; gene expression; positive regulation of transcription from RNA polymerase II promoter; posttranscriptional gene silencing