Goat EIF2C1/AGO1 Polyclonal Antibody | anti-EIF2C1 antibody

Goat anti-EIF2C1/AGO1 Antibody

Cat. Num. AAA421265

• Gene Names: AGO1; Q99; EIF2C; hAgo1; EIF2C1; GERP95
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: EIF2C1/AGO1; Polyclonal Antibody; Goat anti-EIF2C1/AGO1 Antibody; EIF2C1; eukaryotic translation initiation factor 2C; 1; AGO1; EIF2C; GERP95; Q99; Golgi Endoplasmic Reticulum protein 95 kDa; argonaute 1; putative RNA-binding protein Q99; EIF2C1 antibody; 1 antibody; AGO1 antibody; EIF2C antibody; GERP95 antibody; Q99 antibody; Golgi Endoplasmic Reticulum protein 95 kDa antibody; argonaute 1 antibody; putative RNA-binding protein Q99 antibody; EIF2C1 / AGO1; anti-EIF2C1 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: KNASYNLDPYIQEF
• Sequence Length: 857

• Applicable Applications for anti-EIF2C1 antibody: Peptide ELISA (EIA), Western Blot (WB-Trf)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: In transfected HEK293 transiently expressing human EIF2C1bands of approx.110kDa and 35kDa are observed. The 110kDa band is not observed in the non-transfected HEK293. The calculated molecular size is 97.2kDa according to NP_036331.1. Recommended concentration, 0.3-1ug/ml.
• Immunogen: Peptide with sequence C-KNASYNLDPYIQEF, from the internal region of the protein sequence according to NP_036331.1.
• Epitope: Internal region
• Note: This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

(HEK293 overexpressing EIF2C1 and probed (non-transfected HEK293 in lane B).)

NCBI and Uniprot Product Information

• NCBI GI #: 6912352
• NCBI GeneID: 26523
• NCBI Accession #: NP_036331.1
• NCBI GenBank Nucleotide #: NM_012199.4
• Molecular Weight: 97,214 Da
• NCBI Official Full Name: protein argonaute-1 isoform 1
• NCBI Official Synonym Full Names: argonaute 1, RISC catalytic component
• NCBI Official Symbol: AGO1
• NCBI Official Synonym Symbols: Q99; EIF2C; hAgo1; EIF2C1; GERP95
• NCBI Protein Information: protein argonaute-1
• UniProt Protein Name: Protein argonaute-1
• UniProt Gene Name: AGO1
• UniProt Synonym Gene Names: EIF2C1; Argonaute1; hAgo1; eIF-2C 1; eIF2C 1
• UniProt Entry Name: AGO1_HUMAN

NCBI Description

This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]

Uniprot Description

AGO1: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, EIF2C2/AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with LIMD1, WTIP and AJUBA. Belongs to the argonaute family. Ago subfamily.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; polysome; ribonucleoprotein complex

Molecular Function: double-stranded RNA binding; endoribonuclease activity; miRNA binding; protein binding; RNA binding; single-stranded RNA binding

Biological Process: miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; miRNA-mediated gene silencing, production of miRNAs; phosphoinositide-mediated signaling; positive regulation of transcription from RNA polymerase II promoter; pre-microRNA processing; RNA secondary structure unwinding; RNA-mediated gene silencing; RNA-mediated posttranscriptional gene silencing; transcription, DNA-dependent; Wnt receptor signaling pathway, calcium modulating pathway