Rabbit anti-Human EIF2B1 Polyclonal Antibody | anti-EIF2B1 antibody

EIF2B1, ID (EIF2B1, EIF2BA, Translation initiation factor eIF-2B subunit alpha, eIF-2B GDP-GTP exchange factor subunit alpha) (APC)

Cat. Num. AAA6294875

• Gene Names: EIF2B1; EIF2B; EIF2BA
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EIF2B1; Polyclonal Antibody; ID (EIF2B1; EIF2BA; Translation initiation factor eIF-2B subunit alpha; eIF-2B GDP-GTP exchange factor subunit alpha) (APC); eIF-2B GDP-GTP exchange factor subunit alpha; anti-EIF2B1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-EIF2B1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EIF2B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-163 amino acids from the Central region of human EIF2B1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(EIF2B1 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the EIF2B1 antibody detected the EIF2B1 protein (arrow).)

Immunohistochemistry (IHC)

(EIF2B1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF2B1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EIF2B1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-EIF2B1 antibody

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.

Product Categories/Family for anti-EIF2B1 antibody

Antibodies; Translation Initiation Factors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Hiyama, T.B., et al. J. Mol. Biol. 392(4):937-951(2009). Pronk, J., et al. Mult. Scler. 14(8):1123-1126(2008). van der Knaap, M.S., et al. Neuropediatrics 38 (5), 264 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4503503
• NCBI GeneID: 1967
• NCBI Accession #: NP_001405.1
• NCBI GenBank Nucleotide #: NM_001414.3
• UniProt Accession #: Q14232; Q3SXP4; A6NLY9; B4DGX0
• Molecular Weight: 24,618 Da
• NCBI Official Full Name: translation initiation factor eIF-2B subunit alpha
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
• NCBI Official Symbol: EIF2B1
• NCBI Official Synonym Symbols: EIF2B; EIF2BA
• NCBI Protein Information: translation initiation factor eIF-2B subunit alpha; eIF-2B GDP-GTP exchange factor subunit alpha
• UniProt Protein Name: Translation initiation factor eIF-2B subunit alpha
• Protein Family: Translation initiation factor
• UniProt Gene Name: EIF2B1
• UniProt Synonym Gene Names: EIF2BA
• UniProt Entry Name: EI2BA_HUMAN

NCBI Description

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Oct 2009]

Uniprot Description

eIF2B-alpha: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Defects in EIF2B1 are a cause of leukodystrophy with vanishing white matter (VWM). VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. Belongs to the eIF-2B alpha/beta/delta subunits family.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: eukaryotic translation initiation factor 2B complex; membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; GDP binding; GTP binding; translation initiation factor activity; guanyl-nucleotide exchange factor activity; S-methyl-5-thioribose-1-phosphate isomerase activity; enzyme regulator activity

Biological Process: response to peptide hormone stimulus; translation; cellular response to stimulus; cellular protein metabolic process; methionine salvage; response to heat; translational initiation; response to glucose stimulus; negative regulation of translation initiation in response to stress; gene expression; oligodendrocyte development; regulation of translational initiation; positive regulation of GTPase activity

Disease: Leukoencephalopathy With Vanishing White Matter