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Western Blot (WB)
(Western blot analysis of extracts from various samples, using Phospho-eIF2B epsilon (Ser540) Antibody.Lane 1: Hela cells(serum starvation treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(serum starvation treatment),Lane 3: Rat brain.)
Rabbit eIF2B epsilon Polyclonal Antibody | anti-eIF2Bepsilon antibody
Phospho-eIF2B epsilon (Ser540) Antibody
Gene Names
EIF2B5; CLE; CACH; LVWM; EIF-2B; EIF2Bepsilon
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (80%), Bovine (80%), Horse (80%), Rabbit (80%), Dog (80%), Chicken (80%)
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
eIF2B epsilon; Polyclonal Antibody; Phospho-eIF2B epsilon (Ser540) Antibody; CACH; CLE; EI2BE_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor; eIF 2B GDP GTP exchange factor subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon; EIF2B; EIF2B5; EIF2BE; EIF2Bepsilon; Eukaryotic Translation Initiation Factor 2 Beta Epsilon; Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa; Eukaryotic translation initiation factor 2B subunit 5 epsilon; LVWM; Translation initiation factor eIF 2B subunit epsilon; Translation initiation factor eIF-2B subunit epsilon; anti-eIF2Bepsilon antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (80%), Bovine (80%), Horse (80%), Rabbit (80%), Dog (80%), Chicken (80%)
Predicted Reactivity: Pig (80%), Bovine (80%), Horse (80%), Rabbit (80%), Dog (80%), Chicken (80%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-eIF2B epsilon (Ser540) Antibody detects endogenous levels of eIF2B epsilon only when phosphorylated at Ser540.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-eIF2Bepsilon antibody
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human eIF2B-epsilon around the phosphorylation site of Ser540.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated at Ser-544 by DYRK2; this is required for subsequent phosphorylation by GSK3B (By similarity). Phosphorylated on serine and threonine residues by GSK3B; phosphorylation inhibits its function.Polyubiquitinated, probably by NEDD4.
Subunit Structure
Complex of five different subunits; alpha, beta, gamma, delta and epsilon. Interacts with RGS2.
Similarity
Belongs to the eIF-2B gamma/epsilon subunits family.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from various samples, using Phospho-eIF2B epsilon (Ser540) Antibody.Lane 1: Hela cells(serum starvation treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(serum starvation treatment),Lane 3: Rat brain.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using Phospho-eIF2B epsilon (Ser540) Antibody.Lane 1: Hela cells(serum starvation treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(serum starvation treatment),Lane 3: Rat brain.)
Western Blot (WB)
(Western blot analysis of extracts from 293, using eIF2B-ε (Phospho-Ser540) Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293, using eIF2B-ε (Phospho-Ser540) Antibody.)
Western Blot (WB)
(Western blot analysis of eIF2B-ε (Phospho-Ser540) using Na3VO4 treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis of eIF2B-ε (Phospho-Ser540) using Na3VO4 treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Immunohistochemistry (IHC)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-eIF2Bepsilon antibody
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,380 Da
NCBI Official Full Name
translation initiation factor eIF-2B subunit epsilon
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
NCBI Official Symbol
EIF2B5
NCBI Official Synonym Symbols
CLE; CACH; LVWM; EIF-2B; EIF2Bepsilon
NCBI Protein Information
translation initiation factor eIF-2B subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon
UniProt Protein Name
Translation initiation factor eIF-2B subunit epsilon
UniProt Gene Name
EIF2B5
UniProt Synonym Gene Names
EIF2BE
UniProt Entry Name
EI2BE_HUMAN
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Product Notes
The eIF2Bepsilon eif2b5 (Catalog #AAA9614204) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-eIF2B epsilon (Ser540) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (80%), Bovine (80%), Horse (80%), Rabbit (80%), Dog (80%), Chicken (80%) and may cross-react with other species as described in the data sheet. AAA Biotech's eIF2B epsilon can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:1000-3000 IHC: 1:50-1:200 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the eIF2Bepsilon eif2b5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF2B epsilon, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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