Rabbit anti-Human EIF2AK1 Polyclonal Antibody | anti-EIF2AK1 antibody

EIF2AK1, NT (Eukaryotic Translation Initiation Factor 2 alpha Kinase 1, Heme-regulated Eukaryotic Initiation Factor eIF-2-alpha Kinase, Heme-regulated Inhibitor, Heme-controlled Repressor, HCR, Hemin-sensitive Initiation Factor-2 alpha Kinase, PRO1362, HR

Cat. Num. AAA6475166

• Gene Names: EIF2AK1; HCR; HRI
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: EIF2AK1; Polyclonal Antibody; NT (Eukaryotic Translation Initiation Factor 2 alpha Kinase 1; Heme-regulated Eukaryotic Initiation Factor eIF-2-alpha Kinase; Heme-regulated Inhibitor; Heme-controlled Repressor; HCR; Hemin-sensitive Initiation Factor-2 alpha Kinase; PRO1362; HR; anti-EIF2AK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HRI.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-EIF2AK1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human HRI (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-HRI Pab in A375 cell lysate. HRI (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-EIF2AK1 antibody

Antibodies; Translation Initiation Factors

References

1. Rafie-Kolpin, M., et al., Biochemistry 42(21):6536-6544 (2003). 2. Chen, J.J., et al., Trends Biochem. Sci. 20(3):105-108 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 197245437
• NCBI GeneID: 27102
• NCBI Accession #: NP_001127807.1
• NCBI GenBank Nucleotide #: NM_001134335.1
• UniProt Accession #: Q9BQI3; Q549K6; Q8NBW3; Q9HC02; Q9NYE0; Q9P0V6; Q9P1J5; Q9P2H8; Q9UHG4; A8K2R2
• Molecular Weight: 71,106 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 2-alpha kinase 1 isoform b
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 1
• NCBI Official Symbol: EIF2AK1
• NCBI Official Synonym Symbols: HCR; HRI
• NCBI Protein Information: eukaryotic translation initiation factor 2-alpha kinase 1; heme-regulated inhibitor; heme-regulated repressor; heme-controlled repressor; heme sensitive initiation factor 2a kinase; heme regulated initiation factor 2 alpha kinase; heme-regulated initiatio
• UniProt Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 1
• Protein Family: Eukaryotic translation initiation factor 2-alpha kinase
• UniProt Gene Name: EIF2AK1
• UniProt Synonym Gene Names: HRI; KIAA1369; HCR
• UniProt Entry Name: E2AK1_HUMAN

NCBI Description

The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

HRI: a protein kinase of the PEK family. Phosphorylates the alpha subunit of eukaryotic initiation factor 2 in response to various stress conditions, inhibiting protein synthesis at the level of initiation. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. Binding of nitric oxide (NO) to the heme iron in the N-terminal heme-binding domain activates the kinase activity, while binding of carbon monoxide (CO) suppresses kinase activity. Requires association with a multiprotein complex containing Hsp90, CDC37 and PPP5C for maturation and activation by autophosporylation. Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.11.1; Translation; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; PEK family

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cytoplasm

Molecular Function: protein homodimerization activity; heme binding; eukaryotic translation initiation factor 2alpha kinase activity; ATP binding

Biological Process: response to external stimulus; negative regulation of cell proliferation; negative regulation of translational initiation by iron; protoporphyrinogen IX metabolic process; protein amino acid autophosphorylation; response to stress; negative regulation of hemoglobin biosynthetic process