Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse kidney tissue, Primary antibody: 861639(EID3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Rabbit anti-Human, Mouse EID3 Polyclonal Antibody | anti-EID3 antibody
EID3 Rabbit pAb
Gene Names
EID3; NS4EB; NSE4B; NSMCE4B
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
EID3; Polyclonal Antibody; EID3 Rabbit pAb; NS4EB; NSE4B; NSMCE4B; anti-EID3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-EID3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
Immunogen
Synthetic peptide of human EID3
Conjugation
Unconjugated
Molecular Function
EID3 is highly expressed in testis and shows homology to a region of EID1 implicated in binding to CBP/p300. EID3 acts as a potent inhibitor of nuclear receptor transcriptional activity by a mechanism that is independent of direct interactions with nuclear receptors, including SHP. Furthermore, EID3 directly binds to and blocks the SRC-1 interacting domain of CBP, which has been implicated to act as the interaction surface for nuclear receptor co-activators. Consistent with this idea, EID3 prevents recruitment of CBP to a natural nuclear receptor-regulated promoter.
Modification
Unmodified
Cell Position
Chromosome, Cytoplasm, Nucleus, Telomere
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse kidney tissue, Primary antibody: 861639(EID3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse kidney tissue, Primary antibody: 861639(EID3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 861639(EID3 Antibody) at dilution 1/15. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 861639(EID3 Antibody) at dilution 1/15. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 861639(EID3 Antibody) at dilution 1/15. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 861639(EID3 Antibody) at dilution 1/15. (Original magnification: ×200))
Product Categories/Family for anti-EID3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,168 Da
NCBI Official Full Name
EP300-interacting inhibitor of differentiation 3
NCBI Official Synonym Full Names
EP300 interacting inhibitor of differentiation 3
NCBI Official Symbol
EID3
NCBI Official Synonym Symbols
NS4EB; NSE4B; NSMCE4B
NCBI Protein Information
EP300-interacting inhibitor of differentiation 3; E1A-like inhibitor of differentiation 3; EID-1-like inhibitor of differentiation 3; EID-3; EID3; non-SMC element 4 homolog B; non-structural maintenance of chromosomes element 4 homolog B
UniProt Protein Name
EP300-interacting inhibitor of differentiation 3
Protein Family
UniProt Gene Name
EID3
UniProt Synonym Gene Names
EID-3; NS4EB; Non-SMC element 4 homolog B
UniProt Entry Name
EID3_HUMAN
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Product Notes
The EID3 eid3 (Catalog #AAA4758426) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EID3 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EID3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the EID3 eid3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EID3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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