Rabbit EHD1 Polyclonal Antibody | anti-EHD1 antibody

Anti-EHD1 Antibody

Cat. Num. AAA1753497

• Gene Names: EHD1; PAST; PAST1; H-PAST; HPAST1
• Reactivity: Human, Mouse, Monkey
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Immunogen affinity purified.
• Synonyms: EHD1; Polyclonal Antibody; Anti-EHD1 Antibody; PAST; PAST1; CDABP0131; EH domain-containing protein 1; PAST homolog 1; hPAST1; Testilin; EH domain containing 1; anti-EHD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Monkey
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for EHD1 detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• Applicable Applications for anti-EHD1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM)
• Application Notes: WB: 0.25-0.5ug/ml|Human, Mouse, Monkey|; ICC/IF: 5ug/ml|Human|; FC/FACS/FCM: 1-3ug/1x106 cells|Human|
• Immunogen: A synthetic peptide corresponding to a sequence of human EHD1 (HELPADLPPHLVPPSKRRHE).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-EHD1 antibody

EH domain-containing protein 1, also known as testilin or PAST homolog 1 (PAST1), is a protein that in humans is encoded by the EHD1 gene, belonging to the EHD protein family. It is mapped to 11q13. 1. This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene.

References

1. Haider, N. B., Searby, C., Galperin, E., Mintz, L., Horowitz, M., Stone, E. M., Sheffield, V. C. Evaluation and molecular characterization of EHD1, a candidate gene for Bardet-Biedl syndrome 1 (BBS1). Gene 240: 227-232, 1999.
2. Mintz, L., Galperin, E., Pasmanik-Chor, M., Tulzinsky, S., Bromberg, Y., Kozak, C. A., Joyner, A., Fein, A., Horowitz, M. EHD1--an EH-domain-containing protein with a specific expression pattern. Genomics 59: 66-76, 1999.
3. Naslavsky, N., Boehm, M., Backlund, P. S., Jr., Caplan, S. Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Molec. Biol. Cell 15: 2410-2422, 2004.

NCBI and Uniprot Product Information

• NCBI GI #: 30240932
• NCBI GeneID: 10938
• NCBI Accession #: NP_006786.2
• NCBI GenBank Nucleotide #: NM_006795.3
• UniProt Accession #: Q9H4M9; O14611; Q2M3Q4; Q9UNR3
• Molecular Weight: 60,627 Da
• NCBI Official Full Name: EH domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: EH-domain containing 1
• NCBI Official Symbol: EHD1
• NCBI Official Synonym Symbols: PAST; PAST1; H-PAST; HPAST1
• NCBI Protein Information: EH domain-containing protein 1; testilin; PAST homolog 1
• UniProt Protein Name: EH domain-containing protein 1
• Protein Family: EH domain-containing protein
• UniProt Gene Name: EHD1
• UniProt Synonym Gene Names: PAST; PAST1; hPAST1
• UniProt Entry Name: EHD1_HUMAN

NCBI Description

This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

EHD1: Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes.

Protein type: Calcium-binding; Vesicle

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: membrane; recycling endosome membrane; early endosome membrane; plasma membrane; lipid particle; endosome membrane

Molecular Function: protein binding; GTP binding; calcium ion binding; ATP binding; Rab GTPase binding

Biological Process: intracellular protein transport; cholesterol homeostasis; endocytosis; blood coagulation; endocytic recycling; neurite development; protein homooligomerization