Goat EGR4 Polyclonal Antibody | anti-EGR4 antibody

Goat anti-EGR4 Antibody

Cat. Num. AAA421310

• Gene Names: EGR4; NGFIC; NGFI-C; PAT133
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: EGR4; Polyclonal Antibody; Goat anti-EGR4 Antibody; early growth response 4; NGFI-C; NGFIC; PAT133; EGR4 antibody; early growth response 4 antibody; NGFI-C antibody; NGFIC antibody; PAT133 antibody; anti-EGR4 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: NCGSQGDYQAAPE
• Sequence Length: 486

• Applicable Applications for anti-EGR4 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 4000.; Western Blot: Approx 50kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 50.8kDa according to NP_001956.2). Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence NCGSQGDYQAAPE, from the internal region of the protein sequence according to NP_001956.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 145301592
• NCBI GeneID: 1961
• NCBI Accession #: NP_001956.2
• NCBI GenBank Nucleotide #: NM_001965.2
• Molecular Weight: 61,623 Da
• NCBI Official Full Name: early growth response 4
• NCBI Official Synonym Full Names: early growth response 4
• NCBI Official Symbol: EGR4
• NCBI Official Synonym Symbols: NGFIC; NGFI-C; PAT133
• NCBI Protein Information: early growth response protein 4
• UniProt Protein Name: Early growth response protein 4
• Protein Family: Early growth response protein
• UniProt Gene Name: EGR4
• UniProt Synonym Gene Names: EGR-4
• UniProt Entry Name: EGR4_HUMAN

Uniprot Description

EGR4: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. Belongs to the EGR C2H2-type zinc-finger protein family.

Protein type: DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; transcription factor activity

Biological Process: positive regulation of cell proliferation; regulation of transcription, DNA-dependent; transcription, DNA-dependent