EGFR-Y1045 Antibody Pair Kit | EGFR antibody pair kit

Phospho-EGFR-Y1045 Antibody Kit

Cat. Num. AAA9135700

• Gene Names: EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Synonyms: EGFR-Y1045; Polyclonal Antibody Pair Kit; Phospho-EGFR-Y1045 Antibody Kit; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61; EGFR antibody pair kit

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence Length: 1210

• Applicable Applications for EGFR antibody pair kit: Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: A phospho specific peptide corresponding to residues surrounding Y1045 of human EGFR; Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
• Cellular Location: Cell Membrane, Endoplasmic Reticulum Membrane, Endosome, Endosome Membrane, Golgi Apparatus Membrane, Nucleus Membrane, Nucleus, Secreted, Single-pass type I Membrane Protein
• Preparation and Storage: Store at-20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot-Phospho-EGFR-Y1045 pAb)

Western Blot (WB)

(Western blot-[KO Validated] EGFR Polyclonal Antibody)

Western Blot (WB)

(Western blot-[KO Validated] EGFR Polyclonal Antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry-[KO Validated] EGFR Polyclonal Antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry-[KO Validated] EGFR Polyclonal Antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry-[KO Validated] EGFR Polyclonal Antibody)

Immunofluorescence (IF)

(Immunofluorescence-[KO Validated] EGFR Polyclonal Antibody)

Immunofluorescence (IF)

(Immunofluorescence-[KO Validated] EGFR Polyclonal Antibody)

Immunofluorescence (IF)

(Immunofluorescence-[KO Validated] EGFR Polyclonal Antibody)

Immunoprecipitation (IP)

(Immunoprecipitation-[KO Validated] EGFR Polyclonal Antibody)

Related Product Information for EGFR antibody pair kit

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Product Categories/Family for EGFR antibody pair kit

Phosphorylation pAb

NCBI and Uniprot Product Information

• NCBI GI #: 29725609
• NCBI GeneID: 1956
• NCBI Accession #: NP_005219.2
• NCBI GenBank Nucleotide #: NM_005228.5
• UniProt Accession #: P00533; O00688; O00732; P06268; Q14225; Q68GS5; Q92795; Q9BZS2; Q9GZX1; Q9H2C9; Q9H3C9; Q9UMD7
• Molecular Weight: Calculated MW: 44kDa; 69kDa; 77kDa; 134kDa; Observed MW: 180kDa
• NCBI Official Full Name: epidermal growth factor receptor isoform a
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: EGFR
• NCBI Official Synonym Symbols: ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• NCBI Protein Information: epidermal growth factor receptor
• UniProt Protein Name: Epidermal growth factor receptor
• Protein Family: Epidermal growth factor receptor
• UniProt Gene Name: EGFR
• UniProt Synonym Gene Names: ERBB; ERBB1; HER1

NCBI Description

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Uniprot Description

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Plays a role in enhancing learning and memory performance ().